@article {198,
	title = {4,5-diarylisoxazole Hsp90 chaperone inhibitors: potential therapeutic agents for the treatment of cancer},
	journal = {Journal of medicinal chemistry},
	volume = {51},
	year = {2008},
	month = {2008/01/24/},
	pages = {196 - 218},
	abstract = {Inhibitors of the Hsp90 molecular chaperone are showing considerable promise as potential chemotherapeutic agents for cancer. Here, we describe the structure-based design, synthesis, structure-activity relationships and pharmacokinetics of potent small-molecule inhibitors of Hsp90 based on the 4,5-diarylisoxazole scaffold. Analogues from this series have high affinity for Hsp90, as measured in a fluorescence polarization (FP) competitive binding assay, and are active in cancer cell lines where they inhibit proliferation and exhibit a characteristic profile of depletion of oncogenic proteins and concomitant elevation of Hsp72. Compound 40f (VER-52296/NVP-AUY922) is potent in the Hsp90 FP binding assay (IC50 = 21 nM) and inhibits proliferation of various human cancer cell lines in vitro, with GI50 averaging 9 nM. Compound 40f is retained in tumors in vivo when administered i.p., as evaluated by cassette dosing in tumor-bearing mice. In a human colon cancer xenograft model, 40f inhibits tumor growth by approximately 50\%.},
	keywords = {Animals; Antineoplastic Agents/chemical synthesis/pharmacokinetics/pharmacology; Binding, Antitumor; Fluorescence Polarization; HSP90 Heat-Shock Proteins/antagonists \& inhibitors/metabolism; Humans; Isoxazoles/chemical synthesis/pharmacokinetics/pharmacology; Mice; Mice, Competitive; Cell Line, Heterologous, Molecular; Neoplasm Transplantation; Resorcinols/chemical synthesis/pharmacokinetics/pharmacology; Structure-Activity Relationship; Transplantation, Nude; Models, Tumor; Cell Proliferation/drug effects; Crystallography, X-Ray; Drug Screening Assays},
	author = {Brough,P. A. and Aherne,W. and Barril, Xavier and Borgognoni,J. and Boxall,K. and Cansfield,J. E. and Cheung,K. M. and Collins,I. and Davies,N. G. and Drysdale,M. J. and Dymock,B. and Eccles,S. A. and Finch,H. and Fink,A. and Hayes,A. and Howes,R. and Hubbard,R. E. and James,K. and Jordan,A. M. and Lockie,A. and Martins,V. and Massey,A. and Matthews,T. P. and McDonald,E. and Northfield,C. J. and Pearl,L. H. and Prodromou,C. and Ray,S. and Raynaud,F. I. and Roughley,S. D. and Sharp,S. Y. and Surgenor,A. and Walmsley,D. L. and Webb,P. and Wood,M. and Workman,P. and Wright,L.}
}
