DNA-triplex stabilizing properties of 8-aminoguanine

TitleDNA-triplex stabilizing properties of 8-aminoguanine
Publication TypeJournal Article
Year of Publication2000
AuthorsSoliva, R, Garcia GR, Blas JR, Eritja R, Asensio JL, Gonzalez C, Luque FJ, Orozco M
JournalNucleic acids research
Pagination4531 - 4539
Date Published2000/11/15/
KeywordsCytosine/chemistry; DNA/chemistry/genetics; Guanine/analogs & derivatives/chemistry; Magnetic Resonance Spectroscopy/methods; Models, Molecular; Mutation; Nucleic Acid Conformation; Nucleic Acid Denaturation; Structure-Activity Relationship; Thermodynamics
AbstractA DNA-triplex stabilizing purine (8-aminoguanine) is designed based on molecular modeling and synthesized. The substitution of guanine by 8-aminoguanine largely stabilizes the triplex both at neutral and acidic pH, as suggested by molecular dynamics and thermodynamic integration calculations, and demonstrated by melting experiments. NMR experiments confirm the triplex-stabilizing properties of 8-aminoguanine and demonstrate that few changes are found in the structure of the triplex due to the presence of this modified base.