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Measurement of a glycoprotein with blood group A activity by light scattering
Posted June 8th, 2010 by pschmidtke
| Title | Measurement of a glycoprotein with blood group A activity by light scattering |
| Publication Type | Journal Article |
| Year of Publication | 1987 |
| Authors | Estelrich, J, Pouplana R |
| Journal | Journal of Biochemical and Biophysical Methods |
| Volume | 14 |
| Issue | 3 |
| Pagination | 119 - 126 |
| Date Published | 1987/06// |
| Keywords | ABO Blood-Group System; Glycoproteins/blood; Humans; Kinetics; Light; Scattering, Radiation; Spectrophotometry/methods; Thermodynamics |
| Abstract | A light scattering method for quantifying glycoproteins is presented. Conditions of ethanol concentration, wavelength, time of reading, and sample and reagent volumes have been examined. The definitive assay involves addition of 9 vol absolute ethanol to 1 vol sample, mixing, and readings at 105 degrees angle at the interval between 10 and 13 min after placing the cell into the photometer. The obtained values are temperature-dependent. Thus, temperature control is essential. In this assay, a concentration range of 10-60 micrograms glycoprotein has been used. As described, the method is independent of the chemical composition of the different glycoproteins and is barely influenced by size and shape of glycoproteins. |
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