Functional characterization of HERC proteins and their involvment in
neurodegeneration and cancer
Members of the Group:
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J. Luis Rosa, Full professor and Chief of the group
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Eduard Casas, Ph.D. student
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Ouadah Hadjebi, Ph.D. student
-
Irantzu Tato Rodrigo,
Ph.D. student
-
Fabiola Amair Pinedo,
Ph.D. student
-
Bibiana Da Rocha Souto,
Ph.D. student
Research lines:
The main goal our research group is to understand the physiological
function of HERC protein family. This family of proteins contains structural
domains related with the guanine nucleotide exchange factor RCC1 and the
E3-ubiquitin ligases (HECT family). We are focus in the functional characterization
of two members of this family: p532/HERC1 and D25215/HERC3. Previous
studies from our group involved these proteins in vesicle traffic and proliferation.
By this reason, we are studying the role of HERC1 and HERC3 in endocytosis/exocytosis
pathways and in cell proliferation. To understand this function, we are
also analyzing the signal transduction pathways where these proteins are
acting.
Financial support:
“Regulación del tráfico
intracelular por la proteína HERC1 y sus
formas mutadas. El fenotipo tambaleante”.
Proyecto del Ministerio de Educación y Ciencia (BFU2004-06329/BMC)
(2004-2007)
" Caracterización funcional de las
ubiquitina ligasas HERC en modelos
in vivo".
NBFU - Programa Nacional de Biología fundamental Entidad financiadora:
SGPY - Subdirección General de Proyectos de Investigación Número de proyecto:
BFU2007-65452 INICIO/FINALIZACIÓN: 2007/2008
Publications:
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The RCC1 superfamily: from genes, to function, to disease. Hadjebi, O; Casas-Terradellas,
E.; Garcia-Gonzalo, F.R.; Rosa, J.L. (2008) Biochem Biophys Acta-Molecular
Cell Research
(in press)
-
Simultaneous electrophoretic analysis of proteins of very high and low molecular
weights using LAG gels . Casas-Terradellas, E.; Garcia-Gonzalo, F.R.; Hadjebi,
O.; Bartrons, R.; Ventura, F.; Rosa, J.L. (2006)
ELECTROPHORESIS 27,
3935-3938
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TSC1 stabilizes TSC2 by inhibiting the interaction between TSC2 and the
HERC1 ubiquitin ligase. Chong-Kopera H, Inoki K, Li Y, Zhu T, Garcia-Gonzalo
FR, Rosa JL, Guan KL. (2006)
J. Biol. Chem.
281, 8313-8316
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The HERC proteins: functional and evolutionary insights. Garcia-Gonzalo FR,
Rosa JL.
(2005)
Cell Mol
Life Sci. 62:1826-38
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Requirement of
phosphatidylinositol-4,5-bisphosphate for HERC1-mediated guanine nucleotide
release from ARF proteins. Garcia-Gonzalo
FR, Bartrons R, Ventura F, Rosa JL.
(2005) FEBS Lett. Jan 17;579(2):343-8.
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"The giant protein HERC1 is recruited to aluminium fluoride-induced actin-rich
surface protrusions in HeLa cells". Garcia-Gonzalo, F.R.; Muñoz, P.; González,
E.; Casaroli-Marano, R.P.; Vilaró,S.; Bartrons, R.; Ventura, F.; Rosa, J.L.. (2004)
FEBS Letters
; 559:
77-83
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"Abnormal α-synuclein interactions with rab proteins in α-synuclein a30p
transgenic mice." Dalfó, E.; Gómez-Isla, T.; Rosa, J.L.; Barrachina, M.;
Ambrosio, S.; Ferrer, I. (2004) Journal of Neuropathology and Experimental
Neurology. 63:302-313
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"Abnormal α-synuclein interactions with rab3a and rabphilin in diffuse lewy body
disease." Dalfó, E.; Barrachina, M.; Rosa, J.L.; Ambrosio, S.;
Ferrer, I. (2004) Neurobiology of Disease;
16:92-97
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"Interaction between HERC1 and M2-type pyruvate kinase". Garcia-Bonzalo,
F.R.; Cruz, C.; Muñoz, P.; Mazurek, S.; Eigenbrodt, E.; Ventura, F.; Bartrons,
R.; Rosa, J.L. (2003) FEBS Letters 539:78-84
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