Dímero de Fosfofructoquinasa-2 con Fructosa 6-fosfat unida  al centro activo

Miembros del Grupo:

• Dr. Ramon Bartrons Bach , Catedràtico de Bioquímica i Biologia Molecular 
• Dra. Anna Manzano Cuesta, Investigador Postdoctoral
• Dra. Àurea Navarro Sabaté, Investigadora postdoctoral  
• Mercé Obach Cortadellas, Becaria  
• Marta Gómez Martínez, Becaria
• María Nieves Calvo Vidal, Becaria
• Miguel Angel Peña Rico, Becario
• Laura Novellasdemunt Vilaseca

Líneas de trabajo:

• Regulación del metabolismo.
• Isoenzimas de la 6-fosfofructo-2-quinasa/fructosa 2,6-bisfosfatasa. 
• Regulación de la expresión genética por hipoxia, factores de crecimiento y hormonas. 
• Regulación del metabolismo en células cancerosas.

Financiación del Grupo:

1. Regulación de los genes pfkfb3-5 codificantes de los isoenzimas de la 6-fosfofructo-2-quinasa

 SPGC - Programa Nacional de Promoción General del Conocimiento. 2006 a 2009

Investigador Principal: Ramon Bartrons Bach  

Palabras Clave: 003617 - Metabolismo energético / 022429 - Estructura de los genes / 012478 - Expresión de genes clonados / 025101 - Glucolisis/gluconeogénesis

2. Grup de Recerca de Factors de Creixement, Hormones i Metabolisme  

PIGC - Projectes de recerca per potenciar els grups de recerca consolidats  DURS - Departament d'Universitats, Recerca i Societat de la Informació (Generalitat de Catalunya) DURSI 2005  a  2008

Investigador Principal: Ramon Bartrons Bach

Palabras Clave: 019363 - Diabetis / 027092 - Factors de creixement / 027093 - Fetge i metabolisme / 025101 - Glucòlisi/gluconeogènesi / 032710 - Teràpia gènica / 027095 - Microcalorimetria / 027096 - Mitogènesi / 027098 - Apoptosi / 027097 - Regulació enzimàtica / 028494 - Tràfic intracel.lular 

Publicaciones:

• Hypoxia, glucose metabolism and the Warburg’s effect . Bartrons, R.; Caro, J. (2007) J. Bioenergetics and Biomembranes  (en premsa)
• TIGAR, a p53-inducible regulator of glycolysis and apoptosis. Bensaad K, Tsuruta A, Selak MA, Vidal MN, Nakano K, Bartrons R, Gottlieb E, Vousden KH  (2006) Cell 126, 107-120
• Mediators of ischemic hepatic preconditioning after cold preservation identified by microarray analysis.  Navarro-Sabaté, À.; Peralta, C.; Calvo, M. N.; Manzano, A.; Massip, M.; Rosselló-Catafau, J.; Bartrons, R. (2006) Liver transplantation 12, 1615-1625
• PFKFB3 gene silencing decreases glycolysis, induces cell-cycle delay and inhibits anchorage-independent growth in HeLa cells.   M. N.; Bartrons, R.(*); Castaño, E.; Perales, J. C.; Navarro-Sabaté, A.; Manzano, A. (2006) FEBS letters 580, 3308-3314
• Heat shock proteins and mitogen-activated protein kinases in steatotic livers undergoing ischemia-reperfusion: some answers . Massip-Salcedo, M.; Casillas-Ramirez, A.; Franco-Gou, R.; Bartrons, R.; Ben Mosbah, I.; Serafin, A.; Roselló-Catafau, J.; Peralta, C. (2006) Am. J. Pathol. 168, 1474-1485
• Overcoming Diabetes-Induced Hyperglycemia through Inhibition of Hepatic Phosphoenolpyruvate Carboxykinase (GTP) with RNAi . Gómez-Valadés, A. G.; Vidal-Alabró, A.; Molas, M.; Boada, J.; Bermúdez, J.; Bartrons, R.; Perales, J. C. (2006) Molecular Therapy  13, 401-412
• How ischaemic preconditioning protects small liver grafts.  Franco-Gou R, Rosello-Catafau J, Casillas-Ramirez A, Massip-Salcedo M, Rimola A, Calvo N, Bartrons R, Peralta C.(2006) J. Pathol. 208, 62-73
• Adenosine monophosphate-activated protein kinase and nitric oxide in rat steatotic liver transplantation. Carrasco-Chaumel E, Rosello-Catafau J, Bartrons R, Franco-Gou R, Xaus C, Casillas A, Gelpi E, Rodes J, Peralta C. (2005) J. Hepatology  43, 997-1006

• copolymers of poly-L-Lysine with serine and tryptophan form stable DNA vectors: implications for receptor-mediated gene transfer. Gómez-Valadés, A.G.; Molas, M.; Vidal-Alabró, A.; Bermúdez, J.; Bartrons, R.; Perales, J. C. (2005) J. Control Release 102, 277-291
• Assessment of a dual regulatory role for NO in liver regeneration after partial hepatectomy: protection against apoptosis and retardation of hepatocyte proliferation.  Zeini M, Hortelano S, Traves PG, Gomez-Valades AG, Pujol A, Perales JC, Bartrons R, Bosca L. (2005). FASEB J. 19(8):995-7
• Specific expression of pfkfb4 gene in spermatogonia germ cells and analysis of its 5'-flanking region. Gomez M, Manzano A, Navarro-Sabate A, Duran J, Obach M, Perales JC, Bartrons R. (2005) FEBS Lett 579, :357-62.
•  6-Phosphofructo-2-kinase (pfkfb3) gene promoter contains hypoxia-inducible factor-1 binding sites necessary for transactivation in response to hypoxia. Obach M, Navarro-Sabate A, Caro J, Kong X, Duran J, Gomez M, Perales JC, Ventura F, Rosa JL, Bartrons R. (2004) J. Biol. Chem. 279, 53562-70.
• Activation of AMP-dependent protein kinase by hypoxia and hypothermia in the liver of frog Rana perezi. Bartrons M, Ortega E, Obach M, Calvo MN, Navarro-Sabate A, Bartrons R.  (2004) Cryobiology   49, 190-4.
• Stimulators of AMP-activated protein kinase inhibit the respiratory burst in human neutrophils. Alba G, El Bekay R, Alvarez-Maqueda M, Chacon P, Vega A, Monteseirin J, Santa Maria C, Pintado E, Bedoya FJ, Bartrons R, Sobrino F. (2004) FEBS Lett. 573, 219-25.
• Regulation of ubiquitous 6-phosphofructo-2-kinase by the ubiquitin-proteasome proteolytic pathway during myogenic C2C12 cell differentiation Riera, L.; Obach, M.; Navarro, A.; Duran, J.; Perales, J.C.; Viñals, F.; Rosa, J.L.; Ventura, F.; Bartrons, R. FEBS Letters (2003), 550, 23-29.
• Receptor-Mediated gene transfer vectors: Progress towards genetic pharmaceuticals.  Molas, M.; Gómez-Valadés, A.G.; Vidal-Alabró, A.; Miguel-Turu, M., Bermudez, J.; Bartrons, R.; Perales J.C. Current Gene Therapy  (2003), 3,  468- 485.  
• The combination of ischemic preconditioning and liver Bcl-2 overexpression is a suitable strategy to
  prevent liver and lung damage after hepatic ischemia-reperfusion. Peralta C, Perales JC, Bartrons R, Mitchell C, Gilgenkrantz H, Xaus C, Prats N, Fernandez L, Gelpi E, Panes J, Rosello-Catafau J. Am J Pathol  (2002) Jun;160(6):2111-22
• Single-stranded DNA condensed with poly-L-lysine results in nanometric particles that are significantly smaller, more stable in physiological ionic strength fluids and afford higher efficiency of gene delivery than their double-stranded counterparts. Molas M, Bartrons R, Perales JC. Biochim. Biophys Acta (2002) 1572(1):37-44
• Insulin induces PFKFB3 gene expression in HT29 human colon adenocarcinoma cells. Riera L, Manzano A, Navarro-Sabate A, Perales JC, Bartrons R. Biochim Biophys Acta (2002)  Apr 3;1589(2):89-92
• Biological properties of poly-L-lysine-DNA complexes generated by cooperative binding of the polycation. Liu G, Molas M, Grossmann GA, Pasumarthy M, Perales JC, Cooper MJ, Hanson RW.  . J. Biol. Chem. 276:34379-87. (2001)
• Adenosine monophosphate-activated protein kinase mediates the protective effects of ischemic preconditioning on hepatic ischemia-reperfusion injury in the rat. Peralta C, Bartrons R, Serafin A, Blazquez C, Guzman M, Prats N, Xaus C, C utillas B, Gelpi E, Rosello-Catafau J.  Hepatology. 34:1164-1173 (2001)
• The human ubiquitous 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase gene (PFKFB3): promoter characterization and genomic structure. Navarro, A.; Manzano, A.; Riera, L.; Rosa, J.L.; Ventura, F.; Bartrons, R. Gene 488: 74-80.  (2001)
• PFK-2/FBPase-2: maker and breaker of the essential biofactor fructose-2,6-bisphosphate. David A. Okar, Ànna Manzano, Àurea Navarro-Sabatè, Lluìs Riera, Ramon Bartrons and Alex J. Lange. (2001) TIBS 26, 30-35.