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| Oral plasma proteins attenuate gut inflammatory effects induced by S. aureus enterotoxin B challenge in rats |
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| Author(s): Perez-Bosque A (Perez-Bosque, Anna)1,2,3, Miro L (Miro, Lluisa)1,2, Polo J (Polo, Javier)3, Russell L (Russell, Louis)4, Campbell J (Campbell, Joy)4, Weaver E (Weaver, Eric)5, Crenshaw J (Crenshaw, Joe)4, Moreto M (Moreto, Miguel)1,2 |
| Source: LIVESTOCK SCIENCE Volume: 133 Issue: 1-3 Special Issue: Sp. Iss. SI Pages: 242-245 Published: SEP 2010 |
Times Cited: 0 References: 15  Citation Map |
Conference Information: 11th International Symposium on Digestive Physiology of Pigs
Costa Daurada, SPAIN, MAY 20-22, 2009 |
| Abstract: In previous studies we have shown that dietary supplementation with spray-dried porcine plasma proteins (SDP) attenuates the effects of the S. aureus enterotoxin B (SEB) on activation of gut-associated lymphoid tissue (GALT) and increased intestinal permeability. We have now studied if the effects of plasma supplementation correlated with mucosal cytokine release and defensin expression. Wistar-Lewis rats were fed diets supplemented with SDP (8% w/w), with a plasma protein fraction containing 50% Ig (PPF; 1.5% w/w) or with milk proteins (Control diet), Animals were fed experimental diets from weaning (day 21) until day 35 after birth, and on days 32 and 35, were administered SEB (i.p. 0.5 mg/kg) to induce a mild intestinal inflammation. Experimental groups were Control, SEB, SEB-SDP and SEB-PPF. Cytokines were determined by a cytometric bead array assay, LTB4 by ELISA and iNOS and defensins by real time PCR, all at 6 h after SEB administration. Results showed that SEB significantly increased mucosal INF-gamma, TNF-alpha, IL-6 and LTB4 concentration (by 110%, 20%, 30% and 110%, respectively; all P<0.05). SDP and PPF partially attenuated these effects. SEB increased mucosal iNOS expression by 22% (P<0.05) and both plasma protein supplements prevented SEB effects on iNOS expression (both P<0.05). SEB administration also reduced the expression of beta-defensin 1 and cryptdin 4 about 10% (P<0.05) and both supplements significantly prevented these effects (P<0.05). SDP increased the expression of mucosal IL-10 supporting the view that this anti-inflammatory cytokine participates in the mechanism of action of dietary plasma proteins during intestinal inflammation. (C) 2010 Elsevier B.V. All rights reserved. |
| Document Type: Proceedings Paper |
| Language: English |
| Author Keywords: Spray-dried animal plasma; Immunoglobulin concentrate; Intestinal inflammation |
| KeyWords Plus: INTESTINAL-MUCOSA; IMMUNE-RESPONSE; WEANED RATS; DEFENSINS |
| Reprint Address: Perez-Bosque, A (reprint author), Univ Barcelona, Fac Farm, Dept Fisiol, Av Joan XXIII S-N, E-08028 Barcelona, Spain |
Addresses:
1. Univ Barcelona, Dept Fisiol Farm, E-08028 Barcelona, Spain
2. Univ Barcelona, Nutr & Food Safety Res Inst, E-08028 Barcelona, Spain
3. APC Europe SA, Granollers, Spain
4. APC Inc, Ankeny, IA USA
5. Proliant Hlth & Biol, Ankeny, IA USA |
| E-mail Addresses: anna.perez@ub.edu |
| Publisher: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS |
| IDS Number: 686VY |
| ISSN: 1871-1413 |
| DOI: 10.1016/j.livsci.2010.06.076 |
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