1) Genetic and epigenetic control of regeneration

We are exploring the cellular and genetic mechanisms that control regeneration of tissues after injury. Fundamental questions must be answered, such as how the size of lost structures is recovered, how proliferation is controlled, and how cells retain a “memory” that allows genetic programs established during development to be maintained and re-activated during regeneration. In addition to that, the understanding of the molecular details that underlie epigenetic mechanisms will be crucial for the comprehension of the maintenance of developmental decisions in regenerating tissues.

Some of these key questions are addressed using genetic and cellular experimental approaches. As model system we use the regeneration ability of the Drosophila imaginal discs. We also are using DNA microarray technology and genetic designs to decipher the genetic basis of imaginal disc regeneration.



Group members: Albert Carbonell, Xavier Vilana, Cora Bergantiños, Marina Ruiz, Federica Mangione, Dr. Enrique Blanco and Dr. Montserrat Corominas




2) Cell signaling: gene regulatory network in the photoreceptor R7 specification

The goal of this research is to explore the complex gene regulatory networks through which signaling pathways interact to promote cell specification. A single cell resolution model to examine the relationship between cell signaling and cell-fate specification is the development of the R7 photoreceptor in the Drosophila eye. The activation of the Sevenless receptor tyrosine kinase plays a pivotal role in the specification of the R7 cell. Although that pathway has been well characterized, its regulators and links to other signaling pathways are poorly known. We are currently exploring that issue by genetic screenings in combination with genetic interactions and clonal analysis in eye imaginal discs, with the aim to identify genes and networks required to control R7 development.

During development high or low activity of signaling pathways may result in different cellular responses. For example, in the developing eye, high levels of Ras are required to trigger R7 fate. Using the eye imaginal disc, we are examining the transcriptomes of gain- and loss-of –function mutants of the Ras pathway to discover the genetic programs that respond to different levels of Ras pathway activity.



Group members: Isabel Almudí, Natalia Mora, Andrea Mateo, Dr. Sílvia Pérez, Dr. Montserrat Corominas