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We have performed studies of protein folding
processes, protein conformational changes and protein interactions with
molecules of pharmacological, biological or environmental interest. Process
monitoring is carried out using spectroscopic techniques, such as circular
dichroism, fluorescence, molecular absorption, IR or mass spectrometry. The
recorded data are analyzed with multivariate resolution techniques, developed
in our research group.
The biological interest of these studies is complemented by the
methodological approach used to get the process description. Given the high
complexity of biological processes, the strategies used in the experimental
monitoring and interpretation of the information can be extended to the
analysis of processes taking place in environmental or industrial scenarios.
Most relevant publications
1. Detection and resolution of intermediate species in protein unfolfing
and folding processes using fluorescence and circular dichroism
spectroscopies and multivariate curve resolution. Navea, S.; de Juan, A.;
Tauler, R. Analytical Chemistry, 64 (2002) 6031 -6039.
2. Modelling temperature-dependent protein structural transitions by combined
NIR and MIR spectroscopies and multivariate curve resolution. S.Navea, A. de
Juan and R.Tauler. Analytical Chemistry, 2003, 75, 5592-5601.
3. Application of multivariate curve resolution to the temperature-induced
unfolding of alpha-chymotrypsin. Borges, A.; Tauler, R.; de Juan, A.
Analytica Chimica Acta, 544 (2005) 159-166.
4. Chemometrics tools for classification and elucidation of protein secondary
structure from infrared and circular dichroism spectroscopic measurements.
Susana Navea, Romà Tauler, Anna de Juan. Proteins-Structure Function and
Genetics, 63 (2006) 527-541.
5. Monitoring and modelling of protein processes using Mass Spectrometry,
Circular Dichroism and multivariate resolution methods. Navea, S.; Tauler,
R.; de Juan, A. Analytical Chemistry 78 (2006) 4768-4778.
6. pH- and time-dependent hemoglobin transitions: a case study for process
modelling. Muñoz, G.; de Juan, A. Analytica Chimica Acta, 595 (2007) 198-208.
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