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Antonella Consiglio, PhD
..Department: Neural Commitment and Differentiation
..Institution: The Institute of Biomedicine of the University of Barcelona (IBUB)

.........Mailing Address
.........Baldiri Reixac 15-21
.........08028, Barcelona, Spain
.........Tel: +34 934 039842
.........Fax: 34 93402155
............e-mail
:aconsiglio@ibub.pcb.ub.es

 

 


Biography

 

Research Topics

 

Lab Members


Alumni

 

Selected Publications
 

Biography

Antonella Consiglio received her Ph.D. in Cellular and Molecular Biology and Pathology from the Telethon Institute for Gene Therapy (TIGET) at San Raffaele Scientific Institute in 2003 for her work studying the efficiency of lentiviral vectors to target specific regions of the brain in vivo in a murine model of Metachromatic Leukodystrophy with distinguished hematologist Dr. C. Bordignon.

After receiving her Ph.D., she was awarded with a postdoctoral fellowship from the Italian Telethon Foundation to conduct research at the Salk Institute in La Jolla, CA, studying adult neural stem cells and brain regeneration in the laboratory of well-known stem cell researcher Fred H. Gage, Ph.D. She continued her research in stem cell biology and adult neurogenesis as a researcher of the Instituto de Salud Carlos III with Dr. J.C. Belmonte from 2006-2009. During this period, she has mainly focused on the field of pluripotent stem cell biology and neural differentiation. Specifically, she has applied promoter-specific lentiviral vectors to perform lineage-tracing analyses of the progeny of neural stem cells in vivo, thus providing the first direct experimental evidence of their long-term self-renewal and multi-lineage differentiation abilities in adult brains in vivo (Cell Stem Cell, 2007, 1:1-14).
Moreover, Dr. A. Consiglio has participated to the successful derivation of some of the first hES cell lines in Spain and their differentiation toward cardiomyocytes and neurons (CSH Symp Quant Biol, 2008); the generation of the first human iPSC lines in Europe (Nat Biotechnol, 2008), and the first generation of disease-corrected patient-specific iPS cells (Nature 2009; Nat Protoc 2010). She is at the IBUB since Nov. 2009. Her research intends to develope novel strategies to manipulate the differentiation potential of human pluripotent stem cells for use in regenerative medicine.

Research Topics

The recent resurgence in the field of adult neurogenesis presents a window of hope for new treatments and cures for the seemingly once irreparable subject of neurodegenerative disorders. It is now a fact that the birth of neurons in the adult human brain is a continuous biological process in at least two regions: the subgranular zone (SGZ) of the dentate gyrus (DG) of the adult hippocampus.

Our laboratory is interested in adult neuroplasticity and regeneration in the brain. Primary focus is on the residual capacity of the adult nervous system to repair itself. Following the discovery of the neural stem cells in the adult nervous system, emphasis has been on understanding the cellular and molecular mechanisms that control the process of neurogenesis in the adult brain. Importantly we are interested in understanding the function and the potential of these newly born cells in physiology and disease.

Specifically, the main goal pursued in our laboratory is to identify part of the multiple signals that are responsible for endogenous precursor division, differentiation, circuit integration and survival in different regions of the brain, and to validate the possibility to manipulate neural precursors toward therapeutic neuronal repopulation for the cure of different degenerative CNS pathologies. By using several approaches, including exogenously regulated expression, transplantation, LV-mediated siRNA delivery, constitutive, conditional and self-excising LV mediated delivery, the function of several candidates genes are investigated in vivo to explore new strategies for the selective amplification of transduced cells, forced differentiation toward cell lineages or forced migration toward diseases sites.

Moreover, in addition to addressing basic questions of stem cell biology and adult neurogenesis, our research interests include the understanding of the molecular basis of human disease and the potential use of pluripotent stem cells (hES cells and iPS cells) for the repair of acquired degenerative diseases, such as Parkinson's disease, stroke, epilepsy, and inherited metabolic neurodegenerative diseases.

Lab Members

Antonella Consiglio, Principal Investigator

Roger Torrent Juan, PhD student

Carles Calatayud Aristoy, PhD student

Carla Sureda, Master student in Biomedical Research

Sara Tamagno, Master Student in Biomedical Research

Catarina Correia da Cruz Honorio, Master Student in Bioengineering and Nanosystems (Practicum internship)

Ana Costa, Undergraduated Student (Practicum internship)

Alumni

Adriana Sanchez-Danes, PhD

Verònica Casadó, MS

Irene Fernández Carasa, MS

Alice Gervasoni, MS

Selected Publications

Orenstein S.J, Kuo S.-H., Tasset I, Arias E., Koga H., Fernandez Carasa I., Cortes E., Honig L.S., Dauer W., Consiglio A., Raya A., Sulzer D., Cuervo A. M. Interplay of LRRK2 with chaperone-mediated autophagy. Nature Neuroscience, 2013 16(4):394-406

Sanchez-Danes A., Richaud Y., Carballo-Carbajal I., Di Guglielmo C., Giralt A., Canals J.M., Memo M., Alberch J., López-Barneo J., Vila M., Cuervo A.M., Tolosa E., Consiglio A.*,Raya A*. Disease- specific phenotypes in dopamine neurons from human iPS- based models of genetic and sporadic Parkinson’s disease. EMBO Molecular Medicine 2012 4(5):380-95. *Co-corresponding authors

McLenachan S, Menchón C, Raya A, Consiglio A, Edel MJ. Cyclin A1 is critical for setting the pluripotent state and reducing tumorigenicity of iPS cells. Stem Cells Dev., 2012 21(15): 2891-9.

Sanchez-Danes A.*, Consiglio A.*#, Richaud Y., Dehay B., Rodriguez-Pizà I., Bové J., Memo M., Vila M., Raya A. and Izpisua Belmonte J.C.# Efficient generation of A9 midbrain dopaminergic neurons by lentiviral delivery of LMX1A in human embryonic stem cells and iPS cells. Human Gene Therapy, 2012 23(1): 56-69. *Co-authors; #Co-Corresp. authors

Rovetta F., Stacchiotti A., Consiglio A., CadeicM., Grigolato P.G., Lavazza A., Rezzani R. Aleo M.F. ER signalling regulation drives the switch between autophagy and apoptosis in NRK-52E cells exposed to cisplatin. Experimental Cell Research, 2012 318(3):238-50

Menendez S, Camus S, Herreria A, Paramonov I, Batlle Morera L, Collado M, Pekarik V, Maceda I, Edel M, Consiglio A, Sanchez A, Li H, Serrano M, Izpisua Belmonte JC.Increased dosage of tumor suppressors limits the tumorigenicity of iPS cells without affecting their pluripotency. Aging Cell, 2012 11(1):41-50.

Winner B., Jappelli R., Maji S.K., Desplats P.A., Boyer L., Aigner S., Hetzer C., Loher T., Vilar M., Campioni S., Tzitzilonis C., Soragni A., Jessberger S., Mira H., Consiglio A., Pham E., Masliah E., Gage F.H., Riek R. In vivo demonstration that {alpha}-synuclein oligomers are toxic. Proc Natl Acad Sci U S A. 2011 108(10): 4194-9.

Mira H., Andreu Z., Suh H., Lie D.C., Jessberger S., Consiglio A.,Emeterio J., Hortigüela R., Marqués-Torrejón M.A., Nakashima K., Colak D., Götz M., Fariñas I., Gage F.H. Signalling through BMPR-IA regulates quiescence and long-term activity of neural stem cells in the adult hippocampus. Cell Stem Cell 2010, 7(1):78-89.

Edel M.J., Menchon C., Menendez S., Consiglio A., Raya A., Izpisua Belmonte J.C. Rem2 GTPase maintains survival of human embryonic stem cells as well as enhancing reprogramming by regulating p53 and cyclin D1.Genes & Development 2010 15; 24(6): 561-73.

Raya A., Rodríguez-Pizà I., Navarro S., Richaud-Patin Y., Guenechea G., Sánchez-Danés A., Consiglio A., Bueren J.,Izpisúa Belmonte JC. A protocol describing the genetic correction of somatic human cells and subsequent generation of iPS cells. Nature Protocols 2010 5(4): 647-60.

Raya A., Rodríguez-Pizà I., Guenechea G., Vassena R., Navarro S., Barrero M.J., Consiglio A., et al. Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells. Nature 2009 460(7251): 53-9.

Jessberger S., Clark R.E., Broadbent N.J., Clemenson G.D., Consiglio A., Lie C.D., Squire L.R., and Gage F.H. Dentate gyrus-specific knockdown of adult neurogenesis identifies a role for newborn neurons in hippocampus-dependent learning and memory. Learning and Memory, 2009 29;16 (2):146-54.

Aasen T., Raya A., Barrero M.J., Garretta E., Consiglio A., Gonzales F., Vassena R., Bilic J., Edel M., Tiscornia G., Pekarik V., Boue S., Belmonte JC. Generation of induced pluripotent stem cells from human keratinocytes. Nature Biotechnology 2008 26(11): 1276-84.

Raya A., Arán B., Rodríguez-Pizà I., Consiglio A., Barri P., Veiga A., Izpisúa Belmonte JC. Generation of cardiomyocytes from new human embryonic stem cell lines derived from poor-quality blastocysts. Cold Spring Harbor Symposia on Quantitative Biology 2008 73:127-35.

Suh H.*, Consiglio A.*, Ray J., Sawai T., D’Amour K. and Gage F.H. In vivo fate analysis reveals the multipotent and self-renewal capacities of Sox2+ neural stem cells in the adult hippocampus. Cell Stem Cell 2007 1: 1-14. * Co-first authors.

Toni N., Teng M., Bushong E., Aimone J., Consiglio A., Zhao C., van Praag H., Martone M.,Ellisman M., Gage F.H. Synapse formation on neurons born in the adult hippocampus. Nature Neuroscience, 2007 10(6): 727-34.

Consiglio A.*, Martino S., Dolcetta D., Cusella G., Benaglia G., Marchesini S., Déglon N., Aebischer P., Wrabetz L., Severini G.M., Bordignon C. Metabolic correction in oligodendrocytes derived from metachromatic leukodystrophy mouse model by using encapsulated recombinant myoblasts. Journal of Neurological Science 2007 15; 255 (1-2):7-16. *Corresponding author.

Lie D.C,. Colamarino S.A., Song H.J., Desire L., Mira H., Consiglio A., Lein E.S., Jessberger S., Lansford H., Dearie A.R., Gage F.H. Wnt signalling regulates adult hippocampal neurogenesis. Nature 2005 27; 437(7063): 1370-5.

Martino S.*, Consiglio A.*, Cavalieri C., Costanzi E., Tiribuzi R., Severini G. M., Bordignon C., Orlacchio A.  Expression and purification of a human soluble Arylsulfatase A for MLD enzyme replacement therapy. J Biotechnol. 2005 25; 117 (3): 243-51. * Co-first authors.

Consiglio A., Gritti A., Dolcetta D., Follenzi A., Bordignon C., Gage F.H., Vescovi A.L., Naldini L. Robust in vivo gene transfer into adult mammalian neural stem cells by lentiviral vectors.Proc Natl Acad Sci U S A 2004 12; 101 (41): 14835-40.

Farson D., Witt R., McGuiness R., Dull T., Kelly M., Song J., Radeke R., Bukovsky A., Consiglio A.,Naldini L . A new generation stable inducible packaging cell line for lentiviral vectors. Human Gene Therapy 2001 12(8): 981-97.

Consiglio A,. Quattrini A., Martino S., Bensadoun J.C., Dolcetta D., Trojani A. Benaglia G., Marchesini S., Cestari V., Oliverio A., Bordignon C., and Naldini L. In vivo gene therapy of metachromatic leukodystrophy by lentiviral vectors: correction of neuropathology and protection against learning impairments in affected mice. Nature Medicine 2001 7(3): 310-316.

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