MICROBIAL
ENZYMES OF INDUSTRIAL AND ENVIRONMENTAL APPLICATION
Researchers:
Dr. F. I. Javier Pastor Blasco (fpastor@bio.ub.es;
fpastor@ub.edu)
Dra. Pilar Diaz Lucea (pdiaz@bio.ub.es; pdiaz@ub.edu)
Members of the Group:
Dra. Núria Prim
Bosch: Ayudante LRU (nprim@bio.ub.es ; nprim@ub.edu)
Óscar Gallardo
Román: Investigador contratado (ogallard@bio.ub.es)
Cristian Ruiz
Rueda: Investigador contratado (cruiz@bio.ub.es)
Pere Picart
Faiget: Becario FPI (picart@bio.ub.es)
Research:
Identification
and characterization of microbial enzymes of industrial and environmental
application
Molecular
biology of cellulases, xylanases and lipases
Development of
enzymes for clean technologies
Participation in
the Consolidated Research Group of Generalitat de Catalunya
Biodegradation
of xenobiotics and natural products
Projects financed in the last 5 years:
Desarrollo de
enzimas microbianas para el reciclado del papel. CICYT-QUI98-0413-CO2-02.
Tractament
enzimàtic de les fibres. Una aproximació a les tecnologies netes al sector
tèxtil. Generalitat de Catalunya, Departament de Medi Ambient (1998-2000).
Biodegradación de
lignina y hemicelulosa. Aspectos enzimáticos, químicos y moleculares.
Aplicaciones industriales y medioambientales. Xarxa temàtica.
CICYT-BIO98-1841-E; CICYT2002.
Evaluación de
métodos biotecnológicos para el blanqueo de la pasta kraft. Influencia en las
propiedades del papel, en su permanencia y en los efluentes.
PPQ2000-0091-P4-04.
Caracterización de
nuevas celulasas y lipasas microbianas. Evaluación de su aplicación en el
reciclado del papel.
PPQ2001-2161-C02-02.
Síntesis de estólidos
por lipoxigenasas y lipasas microbianas para el aprovechamiento de resíduos
oleaginosos industriales. REN2001-3224/ TECNO.
Biodegradació
de xenobiòtics i productes naturals: Aspectes bàsics i aplicacions a les
tecnologies netes. Generalitat de Catalunya. Renovacions:1997/SGR00473,
1999SGR 00024; 2001SGR00143.
Centre de
Refèrencia en Biotecnologia de la Generalitat de Catalunya (CeRBa).
Participation in
Action COST E-23. European Community
(2000-2005).
Recent Publications:
Blanco A, Diaz P, Zueco J,
Parascandola P, Pastor FIJ (1999) A multidomain xylanase from Bacillus sp.
with a region homologous to thermostabilizing domains of thermophilic enzymes.
Microbiology 145:2163-2171.
Diaz P, Prim N, Pastor FIJ (1999)
Direct
fluorescence-based lipase activity assay. BioTechniques 27, 696-700.
Prim N, Blanco A, Martínez J,
Pastor FIJ, Diaz P (2000). estA, a gene coding for a cell-bound esterase from Paenibacillus
sp. BP-23, is a new member of the bacterial subclass of type B
carboxylesterases. Res Microbiol. 151:303-312.
Soriano M, Blanco A, Diaz P,
Pastor FIJ (2000). An unusual pectate-lyase from a Bacillus sp. with
high activity on pectin: cloning and characterization. Microbiology 146:89-95.
Torres AL, Roncero MB, Colom JF,
Pastor FIJ, Blanco A, Vidal T (2000). Influence of a novel xylanase on fibre surfaces.
Performance on ECF bleaching of Eucalyptus oxygen delignified kraft
pulps. Bioresource Technol. 74:135-140.
Pastor FIJ, Pujol X, Blanco A,
Vidal T, Torres AL, Diaz P (2001). Molecular cloning and characterization of a
multidomain endoglucanase from Paenibacillus sp. BP-23. Evaluation of
its performence in pulp refining. Appl. Microbiol. Biotechnol. 55:61-68.
Prim N, Pastor FIJ, Diaz P (2001).
Cloning and
characterisation of a bacterial cell-bound type B carboxylesterase from Bacillus
sp. BP-7. Current Microbiol. 42:237-240.
Sánchez MM, Prim N, Rández-Gil
F, Pastor FIJ, Diaz P (2002). Engineering of baker’s yeasts, E.coli and Bacillus
hosts for the production of Bacillus subtilis Lipase A. Biotechnol. Bioeng. 78:339-345.
Prim N, Pastor FIJ, Diaz P.
(2002). Zymographic detection of cinnamic acid decarboxylase activity. J.
Microbiol. Methods 51:417-420.
García
O, Torres AL, Colom JF, Pastor FIJ, Díaz P, Vidal T (2002). Effect of
cellulase-assisted refining on the properties of dried and never-dried Eucalyptus
pulp. Cellulose 9:115-125.
Ruiz C, Blanco A, Pastor
FIJ, Díaz P (2002). Analysis of Bacillus megaterium lipolytic system
and cloning of LipA, a novel subfamily I.4 bacterial lipase. FEMS Microbiol.
Lett. 217:263-267.
Gallardo
O, Díaz P, Pastor FIJ (2003) Characterization of a Paenibacillus cell
associated xylanase with high activity on aryl-xylosides. A new subclass of
Family 10 xylanases. Appl. Microbiol. Biotechnol. 61:226-233.
Prim N,
Sánchez M, Ruiz C, Pastor FIJ, Díaz P (2003) Use of
methylumbeliferyl-derivative substrates for lipase activity characterization.
J. Mol. Catal. B-Enzym. 22:339-34.
Pèlach MA, Pastor
FIJ, Puig J, Vilaseca F, Mutjé P (2003) Enzymic
deinking of old newspaper with cellulase. Process Biochem. 38:1063-1067.
Sánchez MM, Pastor
FIJ, Díaz P (2003) Exo-mode of action of
cellobiohydrolase Cel48C from Paenibacillus sp. BP-23. A unique
type of cellulase among Bacillales. Eur. J. Biochem.
270:2913-2919.
Ruiz C, Pastor FIJ,
Díaz P (2003) Isolation and characterization
of Bacillus
sp. BP-6 LipA, a ubiquitous lipase among mesophilic Bacillus species. Lett. Appl. Microbiol. 37:354-359.
Prim N, Pastor FIJ, Diaz, P
(2003) Biochemical
studies on cloned Bacillus sp. BP-7 phenolic acid decarboxylase PadA.
Appl. Microbiol. Biotechnol. 63:51-56.
López D, Colom JF, Vidal T, Pastor FIJ, Torres AL (2003)
Flotation
of xerographic printed paper: a study of the effect of the dispersant,
collector and cellulases on handsheet visual appearance. Appita J. 56(6):449-454.
Gallardo O, Díaz P,
Pastor FIJ (2004) Cloning and characterization of
Xylanase A from the Strain Bacillus sp. BP-7:
Comparison with Alkaline pI-Low Molecular Weight Xylanases of Family 11.
Current Microbiol. 48:276-279.
Ruiz C,
Falcocchio S, Xoxi E, Pastor FIJ, Díaz P, Saso L (2004) Activation and
inhibition of Candida rugosa and Bacillus-related
lipases by saturated fatty acids, evaluated by a new colorimetric microassay.
Biochim. Biophys. Acta 1672:184-19.
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Updated: june 2004