Rosina GIRONES i LLOP
Description: Professor / Researcher
Organizative Unit: Department of Microbiology
Address
Department of Microbiology
Faculty Biology. University of Barcelona
Diagonal, 645
08028 Barcelona, Spain
Localization
Room 18
Floor 0
Faculty of Biology, Annex building
Telephone: +34 93 402 14 83
Fax: +34 93 403 90 47
E-Mail rgirones@ub.edu
Laboratory 8
Floor 0
Faculty of Biology, Annex building
Telephone: +34 93 403 90 43
Fax: +34 93 403 90 47
Educational activities:
- Tenure Professor at the
- Teaching in the Degrees
of: Biology, Food Science and Technology and Biochemistry. Coordinating
the subjects “Virology” and “Diagnostic of viruses and other
microorganisms in foods”.
-Professor in the Master at
the University of Barcelona: “Molecular Biotechnology”,
“Water: Interdisciplinary Analysis and Sustainable Management”,
“Advanced Microbiology” and “Food Development and
innovation”, teaching the following subjects respectively:
“Molecular Diagnosis”, “Emergent Pathogens in Water”,
“Clinic Virology” and “Microorganism and Food Safety”.
Doctoral thesis
supervised:
1. Caracterización molecular de los bacteriófagos de Bacteroides fragilis propuestos como indicadores de contaminación fecal vírica de origen humano
Author:
University: University de Barcelona
Faculty: Faculty of Biology
Y
2. Detección y caracterización de virus patógenos humanos en muestras ambientales y moluscos bivalvos
Author: Sonia Pina Pedrero
University: University de Barcelona
Faculty: Faculty of Biology
Y
3. Estudio de la utilidad potencial de bacteriófagos de bacterias entéricas como microorganismos modelo de contaminación fecal vírica en moluscos bivalvos
Author: Idoia Muniain Mujika
University: University de Barcelona
Faculty: Faculty of Biology
Year: 2002 Qualification: A. Cum laude
4. Detección y caracterización de los poliomavirus presentes en la población a partir de muestras ambientales
Author:
University: University de Barcelona
Faculty: Faculty of Biology
Year: 2003 Qualification: A. Cum laude
5. La contaminació vírica en mol.luscs bivalves: detecció i caracterització
Author:
University: University de Barcelona
Faculty: Faculty of Biology
Year: 2004 Qualification: A. Cum laude
6. Epidemiología molecular del virus de la hepatitis E (VHE) en zonas industrializadas
Author: Pilar Clemente Casares
University: University de Barcelona
Faculty: Faculty of Biology
Y
7. Estudi de l’excreció i estabilitat de prions en el medi
Author: Carlos Maluquer de Motes Porta
University: University de Barcelona
Faculty: Faculty of Biology
Y
8. Análisis de poliomavirus y adenovirus humanos como indicadores de la contaminación vírica de origen humano en agua
Author: Nestor Albiñana
University:
Faculty: Faculty of Biology
Year: 2008 Qualification: A. Cum laude
Research and related activities:
Dr. Rosina Girones leads the research laboratory of “Virus
transmitted by water and foods”, where the main pathogens of concern are
hepatitis A and E viruses (HAV and HEV), enteroviruses,
adenoviruses, noroviruses, polyomaviruses BK , JC and prionic proteins.
The research projects described
bellows are the most important activity in the laboratory. However, the laboratory also collaborate with public administrations and
private industry, theses activities are mainly related to the analysis of viral
infections outbreaks associated to water and/or foods.
The
members of this research group are (left to right, top to bottom in the
picture):
- Dr. Néstor Albiñana-Gimenez, nealbinana@ub.edu
Biologist and associate professor. His work is involved in detection and
quantification of adenovirus and human poliomavirus in drinking water as a
method for evaluating the water purification plant processes and molecular
biology of human polyomaviruses.
- Anna Carratalà, acarratala@ub.edu
Biologist and contracted researcher. Her work to obtain the PhD degree
is mainly about the evaluation of methods towards the analysis of norovirus,
HEV and adenovirus in vegetables, fruits and porcine derived food. She also
works in viral stability and disinfection procedures on several food matrices.
- Byron Calgua de León, byroncalgua@ub.edu Microbiologist and contracted researcher. His works to obtain the PhD
degree are about of the stability and quantification of adenovirus, norovirus
and JC polyomavirus in bathing waters. Also works in
studies on stability and contamination of prions in
the environment.
- Ayalkibet Hundesa, ahundesa@ub.edu Veterinarian and contracted
research. He is working in his PhD studies focused on the trazability
of the origin of faecal contamination in water samples, and the use od specific animal viruses as MST
tools. He also involved in the development of molecular methods to the
detection, quantification and characterization of animal viruses present in the
environment.
-
Dr. Rosina Girones, rgirones@ub.edu Head of the research group.
- Dr. Sílvia Bofill-Mas, sbofill@ub.edu She
obtained her Ph. D in Biology at the
- Jesús Rodríguez Manzano, jesus_rodriguez@ub.edu Biologist and PhD student. His work is focused in the
molecular epidemiology of HAV, HEV and their transmission through the
environment (wastewater) and shellfish. At the same time, he is also doing the
risk assessment studies for these matrixes.
The group is part of:
- “Group
of consolidated research” Catalonia Government “Microbiology of
water related with the health”.
- “European
Network for Environmental and Food Virology (ENVIRONET), COST action
- Catalonian
Network of Reference in Biotechnology.
- Water
Institute at the
- Rosina Girones
represents AENOR in the CEN Commission to develop estandarizable methods for virus detection in foods (CEN/TC 275/WG6/TAG4).
Main research projects and contracts at the present:
- 2005-2008. Catalan Government, (Comissionat per a Universitats i Recerca,
2005SGR 00592.
Development and application of
microbiological methods for environmental studies.
Role: Co-Investigator.
- 2005-2008. Spanish ministry
of Education and Science (AGL2005-07776-C03-02).
Development of molecular methods for the
detection and epidemiological typing of pathogenic human emergent viruses if
interests in food safety.
The goal is to develop and validate methods for
the study of emergent viruses, including adenoviruses, noroviruses, hepatitis
A, hepatitis E and the polyomavirus JCV and to
analyze the epidemiological implications and the specific food-related
transmission mechanisms.
Role: PI.
- 2006-2008. NIH, National
Institute of Allergy and Infectious Diseases. (FBG303717-304876).
Recovery and characterization of HEV strains
form the environment in
The goal is to collaborate in the study the
molecular epidemiology of HEV by analyzing the epidemiologcal
pattern of HEV strains in
Role:PI.
- 2006-2008. AGBAR, funded by
Emergent viruses in water and the
use of polyomaviruses and adenoviruses as indicators of the quality of water.
Collaboration with company AGBAR,
Role: PI.
- 2008-2011. European
Commission. Food, Agriculture and Fisheries, and
Biotechnology. Contract 213178.
Integrated Monitoring and control of foodborne viruses in Europ
The project is organized as a consortium of
Europ
Role: PI .
- 2008-2009. Zentrum für Molekulare Neurobiologie (ZMNH), University of
Hamburg, Germany. Study
directed by Dr. Roland Martin, Director of the Institut
für Neuroimmunologie und klinische MS Forschung (INIMS).
Diagnostic and characterizations of
the JCPyV infections and reactivations in patients with Multiple Sclerosis
undergoing new treatments with immunomodulators.
Role: PI of the project related to viral
studies.
- 2007-2008. Europ
H
Coordinator David Key. There is a consortium of 4
laboratories in
Role: PI
of the virus section.
- 2009-2011. Ministerio de Ciencia
e
Integrated risk analysis
using molecular methods of microbial emergent pathogens in water and food:
stability, disinfection and source tracking.
The study will be developed
by a multidisciplinary t
Role: PI and coordinator of the
project.
- 2009-2012. Europ
Project reference: EU FW7 222887-2.
Protecting the food chain from prions:
shaping European priorities through basic and applied research (Priority).
Role: IP of the partner at the
Completed research
support of the last 10 years:
-1997-2003.
The goal of this study is to study the
potential infection of SV40 in humans, isolating SV40 strains from urban sewage
of wide divergent geographical areas.
Role: PI.
-1998-2001. FAIR-CT98-4039.
European Union, Program: AGRI - Agriculture and
agro-industry including fisheries.
Development of techniques for
monitoring and control of human viral contamination of shellfish.
Coordinator of
these studies with collaboration of laboratories of four different countries in
Europe, including the
Role: PI and Coordinator.
- 1998-2001. European Union, Program: COPE -
INCO-COPERNICUS (IC18CT98 0282 ).
Evaluation of the usefulness of bacteriophages as model micro organisms for the assessment
of water treatment process and water quality.
Role: Co-investigator.
- 2000-2002. Spanish Ministry
of Sanity and Consumption (FIS 00/1123).
Identification of new strains of the
virus of hepatitis E (HEV) causing acute hepatitis and of their potential
animal reservoirs.
Collaboration with Hospital Vall d’Hebron in
Role: PI.
- 2002-2006. Spanish Ministry
of Science and Technology (EET2001-4814-C02-01).
Excretion and transmission of prionic proteins through sewage water and fecal contamination.
The goal of this study has been the evaluation of
the excretion by animals or humans of prions and
their potential accumulation in sewage or sludge. Collaboration with the Center for res
Role: PI and Coordinator.
- 2004-2006. Foundation
AGBAR. FBG 303553.
Emergent viruses in water and the
use of polyomaviruses and adenoviruses as indicators of the quality of water.
Role: PI.
- 2005-2007. European
Commission, Policy-oriented research, (513648 (SSPI)).
Methods for the concentration and detection of
adenoviruses and noroviruses in European bathing waters with reference to the
revision of the bathing water directive 76/160/EEC -VIROBATHE-.
Role: PI of the
Selected bibliography of last 10 years:
Pina, S., M. Puig, F. Lucena, J.
Jofre, R. Girones. 1998. Viral pollution in the environment and in shellfish; detection of
human adenovirus by PCR as an index of human viruses. Applied and Environmental
Microbiology, 64(9):3376-3382.
Pina S., A. Creus, N.González, R. Girones, M.
Pina S., J. Jofre, S.U.
Emerson, R.H. Purcell, R. Girones. 1998.
Characterization of a strain of infectious hepatitis E virus isolated from
sewage in an ar
Puig M., R. Girones.
1999. Genomic structure of a phage B40-8 of Bacteroides
fragilis. Microbiology, 145: 1661-1670.
Sommaruga R., B. Sattler, A. Oberleiter, A. Willw., S. Wögrath-Sommaruga, R. Psenner, M.
Bofill-Mas
S., S. Pina, R. Girones. 2000. Documenting the epidemiologic patterns of
polyomaviruses in human populations by studying their presence in urban sewage.
Applied and Environmental Microbiology, 66(1): 238-245.
Puig M., J. Jofre,
R. Girones. 2000. Detection of phages infecting Bacteroides fragilis HSP40 using
a specific DNA probe. Journal of Virological Methods,
88: 163-173.
Pina S., M. Buti,
M. Cotrina, J. Piella, R. Girones. 2000. HEV identified in serum from humans with
acute hepatitis and in sewage of animal origin in
Puig M., S. Pina, F. Lucena, J.
Jofre, R. Girones. 2000. Description of a DNA
amplification procedure for the detection of bacteriophages
of Bacteroides fragilis
HSP40 in environmental samples. Journal of Virological
Methods, 89:159-166.
Muniain-Mujika I., R. Girones, F.
Lucena. 2000. Viral contamination of shellfish: evaluation of methods and
analysis of bacteriophages and human viruses. Journal
of Virological Methods, 89: 109-118.
H.T. Agostini,
A. Deckhut, D. V. Jobes, R.
Girones, G. Schlunck, M.G. Prost, C. Frias, E. Pérez-Trallero, C.F. Ryschkewitsch
and G. L. Stoner. 2001. Genotypes of JC Virus in East, Central and
Bofill-Mas, S., R. Girones. 2001. Excretion and transmission of JCV in human populations. Journal of. Neurovirology, 7(4): 345-349.
Bofill-Mas, S., M. Formiga-Cruz, P. Clemente-Casares, F. Calafell,
R. Girones. 2001. Potential Transmisión of Human
Pina S., Buti M., Jardí R.,
Clemente–Casares P. Jofre J., Girones R. 2001. Genetic analysis of the
hepatitis A virus strains recovered from the environment and from acute
hepatitis patients. Journal of General Virology.
82:2955-2963.
Muniain-Mujika, I., Girones, R., Tofiño-Quesada, G., Calvo, M., Lucena, F. 2002. Depuration
dynamics of viruses in shellfish. International Journal of Food Microbiology,
77:125-133.
Hernroth, B.E., Conden-Hansson,
A-C., Rehnstam-Holm, A-S., S., Girones,
R., Allard A.K. 2002. Environmental Factors Influencing Human Viral Pathogens and Their
Potential Indicator Organisms in the Blue Mussel, Mytilus
edulis: the First Scandinavian Report. Applied and
Environmental Microbiology, 68 (9): 4523-4533.
Formiga-Cruz,
M., Tofiño-Quesada, G., Bofill-Mas, S., Lees, D.N., Henshilwood,
K., Allard A.K., Condin-Hansson, A-C., Hernroth, B.E., Vantarakis, A., Tsibouxi, A., Papapetropoulou,
M., Furones, D., Girones,
R. 2002. Distribution of human viral contamination in
shellfish from different growing areas in
Formiga-Cruz, M., Allard A.K., Condin-Hansson, A-C., Henshilwood,
K., Hernroth, B.E., Jofre,
J., Lees, D.N., Lucena, F., Papapetropoulou,
M., Rangdale, R.E., A., Tsibouxi,
37. Vantarakis, A., Girones, R. 2003.
Evaluation of Potential Indicators of Viral Contamination in
Shellfish with Applicability to Diverse Geographical Areas. Applied and
Environmental Microbiology, 69(3):1556-1563.
Muniain-Mujika,
Bofill-Mas, S., Girones, R. 2003. Role of the Environment in
the Transmission of JCV. Journal of Neurovirology,
9(Suppl.1):54-58.
Clemente-Casares,
P., Pina, S., Buti, M., Jardi, R., Martín, M., Bofill-Mas, S., Girones, R. 2003.
Epidemiological pattern of Hepatitis E Virus infection in
industrialized countries. Emerging Infectious Diseases
9(4):448-454.
Bofill-Mas,
S., Clemente-Casares, P., Major, E.O., Curfman, B., Girones,
R. 2003. Analysis of the excreted JC Virus strains and their
potential oral transmission. Journal of Neurovirology,
9(4):498-507.
Maluquer
de Motes, C., Clemente-Casares, P., Hundesa, A.,
Martín, M., Girones, R. 2003. Detection of bovine and porcine
adenovirus in fecal and environmental samples. Applied and Environmental Microbiology, 70:
1448-1454.
Banks M., G.S. H
Brion, G.,
S. Lingeriddy, T.R. Neelakantan, M. Wang, R. Girones, D. Lees, A. Allard, A.
Vantarakis. 2004. Probing Norwalk-like virus presence in shellfish with
artificial neural networks. Water, Science and Technology, 50(1):125-9.
Bofill-Mas,
S., N. Albiñana-Giménez, P.A. Pipkin, P.D. Minor, R. Girones. 2004. Isolation
of SV40 from the environment of a colony of cynomolgus
monkeys naturally infected with the virus. Virology, 330:1-7.
Buti, M., Clemente-Casares, P., Jardí, R., Formiga-Cruz, M., Schaper, M., Valdes, A.,
Rodríguez-Frias, F., Esteban, R., Girones
R. 2004. Sporadic
cases of acute autochthonous hepatitis E in
Formiga-Cruz, M.; Hundesa, A.; Clemente-Casares, P.; Albiñana-Giménez, N.; Allard, A.; Girones, R. 2005. Nested Multiplex PCR assay for detection of
human enteric viruses in shellfish and sewage. Journal of Virological
Methods, 125:111-115.
Bofill-Mas,
S; Clemente-Casares, P., Albinana-Giménez, N., Maluquer de Motes Porta, C.,
Hundesa Gonfa, A., Girones, R. 2005. Efectos sobre la salud de la
contaminación de agua y alimentos por virus emergentes humanos. Rev. Esp. Salud
Pública, 79:253-269.
Girones,
R. 2005. Human viruses in the environment. Editorial.
Brion G,
Viswanathan C,
Neelakantan TR,
Lingireddy S,
Girones R,
Lees D, Allard A, Vantarakis A. 2005. Artificial neural network prediction of
viruses in shellfish. Appl Environ Microbiol.. 71(9):5244-53.
Girones R. Tracking
Viruses That Contaminate Environments. 2006. Microbe, 1 (1). (American Society for Microbiology).
Albiñana-Gimenez N, Clemente-Casares P, Bofill-Mas S, Hundesa A, Ribas F, Girones R. Distribution of human polyomaviruses,
adenoviruses, and hepatitis E virus in the environment and in a drinking-water
tr
Hundesa A, Maluquer
de Motes C, Bofill-Mas S, Albiñana-Gimenez N, Girones R. 2006. Identification of human and animal adenoviruses
and polyomaviruses for determination of sources of fecal
contamination in the environment. Appl Environ
Microbiol. 72(12):7886-93.
Bofill-Mas S, Albiñana-Gimenez N,
Clemente-Casares P, Hundesa A, Rodriguez-Manzano
J, Allard A, Calvo M, Girones
R. 2006. Quantification
and stability of human adenoviruses and polyomavirus
JCPyV in wastewater matrices. Appl Environ. Microbiol.72(12):7894-6.
Maluquer
de Motes, C., Cano,
M. J., Torres, J. M., Pumarola M., and Girones, R.
2008. Detection and survival of prion
agents in aquatic environments. Water Res.,
42( 10-11):2465-72.
Maluquer
de Motes, C., Grassi, J.,
Simon, S., Herva,
M. E., Torres, J. M., Pumarola M., and Girones, R. 2008. Excretion of BSE and
scrapie prions in stools from murine
models. Veterinary Microbiology, 131(1-2):205-11.
Maluquer de Motes, C.; Simon, S.; Grassi, J.; Torres, J.; Pumarola,
M.; Girones, R. 2008. Assessing the presence of
BSE and scrapie in slaughterhouse wastewater. Journal of Applied Microbiology.
105: 1649-1657.
B. Calgua, A. Mengewein, A. Grünert, S. Bofill-Mas, P. Clemente-Casares, A. Hundesa, A.P. Wyn-Jones, J.M. López-Pila, R. Girones.2008. Development and application of a one-step low cost procedure to concentrate viruses from seawater samples. J Virol Methods. 153: 79-83.
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