Departament de Microbiologia 


Francisco Lucena
Anicet R. Blanch
Cristina Garcia
Joan Jofre
Maite Muniesa





Imamovic L, Martínez-Castillo A, Benavides C, Muniesa M. Envelope stress response BaeSR protects against lysogenic conversion with Shiga toxin 2-encoding phages. Infect Immun. 2015. In press.

Brown-Jaque M, Calero-Cáceres W, Muniesa M. Transfer of antibiotic-resistance genes via phage-related mobile elements. Plasmid. 2015. In press.

Casanovas-Massana A, Gómez-Doñate M, Sánchez D, Belanche-Muñoz LA, Muniesa M, Blanch AR. Predicting fecal sources in waters with diverse pollution loads using general and molecular host-specific indicators and applying machine learning methods. J Environ Manage. 2015. In press.

Quirós P, Martínez-Castillo A, Muniesa M. Improving detection of Shiga toxin-producing Escherichia coli by molecular methods by reducing the interference of free Shiga toxin-encoding bacteriophages. 2014. Appl Environ Microbiol. 1;81(1):415-21.

Muniesa, M; Jofre, J. Identifying and analyzing bacteriophages in human fecal samples. What could we discover? 2014. Future Microbiology. 9: 879-86.

Franz, E.; Delaquis, P.; Morabito, S.; Beutin, L.; Gobius, K.; Rasko, D.; Bono, J.; French, N.; Osek J.; Lindstedt, B-A; Muniesa, et al. Exploiting the explosion of information associated with whole genome sequencing to tackle Shiga toxin-producing Escherichia coli (STEC) in global food production systems. 2014. International Journal of Food Microbiology. 187:57-72

Calero-Caceres, W.; Melgarejo, A.; Stoll, C.; Colomer, M.; Lucena, F.; Jofre, J.; Muniesa, M. Sludge as a potential important source of antibiotic resistance genes in both the bacterial and bacteriophage fraction. 2014. Environmental Science & Technology. 48: 7602-11

Marta Colomer-Lluch, William Calero-Cáceres, Sihem Jebri, Fatma Hmaied, Maite Muniesa and Juan Jofre. Antibiotic resistance genes in bacterial and bacteriophage fractions of Tunisian and Spanish wastewaters as markers to compare the antibiotic resistance patterns in each population. 2014.

Martínez-Castillo A, Muniesa M. Implications of free Shiga toxin-converting bacteriophages occurring outside bacteria for the evolution and the detection of Shiga toxin-producing Escherichia coli. Front Cell Infect Microbiol. 2014 Apr 16;4:46.

Jofre J, Blanch AR, Lucena F, Muniesa M. Bacteriophages infecting Bacteroides as a marker for microbial source tracking. Water Res. 2014 May 15;55:1-11.

Allué-Guardia A, Martínez-Castillo A, Muniesa M. Persistence of infectious Shiga toxin-encoding bacteriophages after disinfection treatments. Appl Environ Microbiol. 2014 Apr;80(7):2142-9.

Colomer-Lluch, M.; Jofre, J.; Muniesa, M. Quinolone resistance genes (qnrA and qnrS) in bacteriophage particles from wastewater samples and the effect of inducing agents on packaged antibiotic resistance genes. 2014. Journal of Antimicrobial Chemotherapy. 5: 1265-

Quirós, P.; Colomer-Lluch, M.; Martínez-Castillo, A.; Miró, E.; Argente, M.; Jofre, J.; Navarro, F.; Muniesa, M. Antibiotic-resistance genes in the bacteriophage DNA fraction of human fecal samples. 2014. Antimicrobial Agents and Chemotherapy. 58: 606-9

Could bacteriophages transfer antibiotic resistance genes from environmental bacteria to human-body associated bacterial populations? Mob Genet Elements. 2013 3(4):e25847.

M Muniesa, A Allué-Guardia, A Martínez-Castillo. Bacteriophage-driven emergence of novel pathogens. Future Virology. 2013. 8 (4), 323-325

Allué-Guardia A, Imamovic L, Muniesa M. Evolution of a Self-Inducible Cytolethal Distending Toxin Type V-Encoding Bacteriophage from Escherichia coli O157:H7 to Shigella sonnei. J Virol. 2013 Dec;87(24):13665-75.

Martinez-Castillo A, Quirós P, Navarro F, Miró E, Muniesa M. Shiga toxin 2-encoding bacteriophages in human fecal samples from healthy individuals. Appl Environ Microbiol. 2013 Aug;79(16):4862-8.

Muniesa M, Colomer-Lluch M, Jofre J. Potential impact of environmental bacteriophages in spreading antibiotic resistance genes. Future Microbiol. 2013 Jun;8(6):739-51.

Colomer-Lluch, M, Mora A., López C., Mamani R., Dahbi G., Marzoa J., Herrera A., Blanco JE, Blanco M, Alonso MP, Jofre J, Muniesa M., Blanco J. 2013. Detection of quinolone-resistant E. coli strains belonging to clonal groups O25b:H4-B2-ST131 and O25b:H4-D-ST69 with high virulence gene content in raw sewage and river water in Barcelona, Spain. J. Antimicrob. Chemother. 2013 68(4):758-65.

Muniesa M, Lucena F, Blanch AR, Payán A, Jofre J. 2012. Use of abundance ratios of somatic coliphages and bacteriophages of Bacteroides thetaiotaomicron GA17 for microbial source identification. Water Res. 2012. 1;46(19):6410-8.

Allué-Guardia A, Jofre J, Muniesa M. 2012. Stability and infectivity of cytolethal distending toxin type V gene-carrying bacteriophages in a water mesocosm and under different inactivation conditions. Appl Environ Microbiol. 78(16):5818-23.

Gómez-Doñate M, Ballesté E, Muniesa M, Blanch AR. 2012. New molecular quantitative PCR assay for detection of host-specific Bifidobacteriaceae suitable for microbial source tracking. Appl Environ Microbiol. 78:5788-95

Muniesa M, Hammerl JA, Hertwig S, Appel B, Brüssow H. 2012. Shiga toxin-producing E. coli O104:H4: a new challenge for microbiology. Appl Environ Microbiol. 78(12):4065-73.

Martínez-Castillo, A.; Allué-Guardia, A.; Dahbi, G.; Blanco, J.; Creuzburg, K.; Schmidt, H.; Muniesa, M. 2012. Type III effector genes and other virulence factors in shiga toxin-producing E. coli isolated from wastewater. Environmental Microbiology Reports. 4: 147-155.

Imamovic L, Muniesa M. 2012. Characterizing RecA-independent induction of Shiga toxin2-encoding phages by EDTA treatment. PLoS One. 2012;7(2):e32393.

Colomer-Lluch, M, L. Imamovic, J. Jofre and M. Muniesa. 2011. Bacteriophages carrying antibiotic resistance genes in fecal wastes from cattle, pigs and poultry. Antimicrob. Agents. Chemother. 55:4908-11.

Gómez-Doñate M, Payán A, Cortés I, Blanch AR, Lucena F, Jofre J, Muniesa M. 2011. Isolation of bacteriophage host strains of Bacteroides species suitable for tracking sources of animal faecal pollution in water. Environ Microbiol. 13:1622-31.

Allué-Guardia A, García-Aljaro C, Muniesa M. 2011. Bacteriophage-encoding cytolethal distending toxin type V gene induced from nonclinical E. coli isolates. Infect Immun. 2011 Aug;79(8):3262-72.

Colomer-Lluch M, Jofre J, Muniesa M. 2011. Antibiotic resistance genes in the bacteriophage DNA fraction of environmental samples. PLoS One. 6(3):e17549.

Imamovic, L., E. Ballesté, J. Jofre and M. Muniesa. 2010. Shiga-toxin converting bacteriophages in wastewater and fecal samples quantified by QPCR. Appl Environ Microbiol. 76:5693-5701

Imamovic L, Jofre J, Schmidt H, Serra-Moreno R, Muniesa M. 2009. Phage-mediated Shiga toxin 2 gene transfer in food and water. Appl Environ Microbiol.75(6):1764-1768.

Imamovic L, Serra-Moreno R, Jofre J, Muniesa M. 2010.Quantification of Shiga toxin 2-encoding bacteriophages, by real-time PCR and correlation with phage infectivity. J Appl Microbiol. 2010 Mar;108(3):1105-14.

Muniesa M, Payan A, Moce-Llivina L, Blanch AR, Jofre J. 2009. Differential persistence of F-specific RNA phage subgroups hinders their use as single tracers for faecal source tracking in surface water. Water Res. 43(6):1559-64.

García-Aljaro C, Muniesa M, Jofre J, Blanch AR. 2009. Genotypic and phenotypic diversity among induced, stx2-carrying bacteriophages from environmental E. coli strains. Appl Environ Microbiol. 75:329-36.

Sekse C, Muniesa M, Wasteson Y. 2008.Conserved Stx2 phages from E. coli O103:H25 isolated from patients suffering from hemolytic uremic syndrome. Foodborne Pathog Dis5(6):801-10.

Serra-Moreno R, Jofre J, Muniesa M. 2008. The CI repressors of Shiga toxin-converting prophages are involved in coinfection of Escherichia coli strains, which causes a down regulation in the production of Shiga toxin 2. J Bacteriol. 190(13):4722-35.

Serra-Moreno R, Jofre J, Muniesa M. 2007. Insertion site occupancy by stx2 bacteriophages depends on the locus availability of the host strain chromosome. J Bacteriol. 189(18):6645-54.

Ebdon J, Muniesa M, Taylor H. 2007. The application of a recently isolated strain of Bacteroides(GB-124) to identify human sources of faecal pollution in a temperate river catchment.Water Res. 41:3683-90.

Muniesa M, Jofre J, García-Aljaro C, Blanch AR. 2006. Occurrence of Escherichia coli O157:H7 and other enterohemorrhagic Escherichia coli in the environment. Environ Sci Technol. 40(23):7141-9.

Serra-Moreno R, Acosta S, Hernalsteens JP, Jofre J, Muniesa M. 2006. Use of the lambda Red recombinase system to produce recombinant prophages carrying antibiotic resistance genes. BMC Mol Biol. 7:31.

Blanch AR, Belanche-Muñoz L, Bonjoch X, et al. 2006. Integrated analysis of established and novel microbial and chemical methods for microbial source tracking. Appl Environ Microbiol.;72(9):5915-26.

Muniesa M, Schembri MA, Hauf N, Chakraborty T. 2006. Active genetic elements present in the locus of enterocyte effacement in E. coli O26 and their role in mobility. Infect Immun. 74:4190-9.

Mesa RJ, Blanc V, et al. Extended-spectrum beta-lactamase-producing Enterobacteriaceae in different environments (humans, food, animal farms and sewage). 2006. J Antimicrob Chemother. 58:211-5.

Payan A, Ebdon J, Taylor H, Gantzer C, Ottoson J, Papageorgiou GT, Blanch AR, Lucena F, Jofre J, Muniesa M. 2005. Method for isolation of Bacteroides bacteriophage host strains suitable for tracking sources of fecal pollution in water. Appl Environ Microbiol. 2005 Sep;71(9):5659-62.

Muniesa M, Blanch AR, Lucena F, Jofre J. 2005. Bacteriophages may bias outcome of bacterial enrichment cultures. Appl Environ Microbiol. 71(8):4269-75.

Muniesa M, Serra-Moreno R, Jofre J. 2004. Free Shiga toxin bacteriophages isolated from sewage showed diversity although the stx genes appeared conserved. Environ Microbiol. 2004

Muniesa M, García A, Miró E, Mirelis B, Prats G, Jofre J, Navarro F. 2004.Bacteriophages and diffusion of beta-lactamase genes. Emerg Infect Dis. Jun;10(6):1134-7.

Muniesa M, Blanco JE, De Simón M, Serra-Moreno R, Blanch AR, Jofre J. 2004. Diversity of stx2 converting bacteriophages induced from Shiga-toxin-producing Escherichia coli strains isolated from cattle. Microbiology. 150:2959-71.

García-Aljaro C, Muniesa M, Jofre J, Blanch AR. 2004. Prevalence of the stx2 gene in coliform populations from aquatic environments. Appl Environ Microbiol;70(6):3535-40.

Muniesa M, de Simon M, Prats G, Ferrer D, Pañella H, Jofre J. 2003. Shiga toxin 2-converting bacteriophages associated with clonal variability in E. coli O157:H7 strains of human origin isolated from a single outbreak. Infect Immun. 2003 71:4554-62.

Durán AE, Muniesa M, Mocé-Llivina L, Campos C, Jofre J, Lucena F. 2003.Usefulness of different groups of bacteriophages as model micro-organisms for evaluating chlorination. J Appl Microbiol.;95:29-37.

Mocé-Llivina L, Muniesa M, Pimenta-Vale H, Lucena F, Jofre J. 2003. Survival of bacterial indicator species and bacteriophages after thermal treatment of sludge and sewage. Appl Environ Microbiol. 69:1452-6.

Mooijman KA, Bahar M, Muniesa M, Havelaar AH. 2002. Optimisation of ISO 10705-1 on enumeration of F-specific bacteriophages. J Virol Methods. 103(2):129-36.

Durán AE, Muniesa M, Méndez X, Valero F, Lucena F, Jofre J. 2002. Removal and inactivation of indicator bacteriophages in fresh waters. J Appl Microbiol. 92(2):338-47.

Araujo R, Muniesa M, Méndez J, Puig A, Queralt N, Lucena F, Jofre J. 2001. Optimisation and standardisation of a method for detecting and enumerating bacteriophages infecting Bacteroides fragilis. J Virol Methods. 93(1-2):127-36.

Muniesa M, Recktenwald J, Bielaszewska M, Karch H, Schmidt H. 2000.Characterization of a shiga toxin 2e-converting bacteriophage from an E. coli strain of human origin. Infect Immun. 68:4850-5.

Muniesa M, Jofre J. 2000. Occurrence of phages infecting E. coli O157:H7 carrying the Stx 2 gene in sewage from different countries. FEMS Microbiol LettFeb 1;183(1):197-200.

Muniesa M, Lucena F, Jofre J. 1999.Comparative survival of free shiga toxin 2-encoding phages and E. coli strains outside the gut. Appl Environ Microbiol. Dec;65(12):5615-8.

Muniesa M, Jofre J. 1998. Abundance in sewage of bacteriophages that infect Escherichia coli O157:H7 and that carry the Shiga toxin 2 gene. Appl Environ Microbiol. 1998 Jul;64(7):2443-8.

Book chapters

Muniesa, M and Scmidt, H. 2013. Shiga Toxin-Encoding Phages -Multifunctional Gene Ferries. Horizon Scientific Press. Book chapter

Muniesa, M. 2010. El bacteriòfag lambda (Bacteriophage lambda). Organismes model en biologia Societat Catalana de Biologia (SCB) Book chapter.

Serra-Moreno, R..; Muniesa, M. 2010. DNA damage-mediated amplification of toxin síntesis. DNA Repair and Bacterial Virulence. Horizon Scientific Press. Book chapter

Muniesa, M. 2009. Bacteriófagos portadores del gen de la Stx 2 en aguas (Bacteriophages carrying Stx2 gene in water). Tesi doctoral en xarxa TDX. Tesis Doctorals en Xarxa. UB.

Muniesa, M.; Serra-Moreno, R.; Jofre, R. 2009. Prophages within the bacterial genomes: Contribution to bacterial variability. Contemporary Trends in Bacteriophage Research. Nova Science Publishers, Inc. Book chapter

Jofre, J.; Muniesa, M. 2004. Abundance in sewage of bacteriophages infecting E. coli O157:H7. Methods in Molecular Biology. Public Health Microbiology. Methods and Protocols. The Humana Press Inc. 268:79-88. ISBN: 978-1-58829-117-2. Book chapter

J. Jofre, M. Muniesa. 1999. Potential use of phages and/or lysins. Encyclopedia of Food Microbiology. R.K. Robinson, C.A. Batt and P.D. Patel Ed. Academic Press. London. pp. 1469-1475. 268:79-88