Stem Cells and Neurodegeneration
Stem Cells and Neurodegeneration Unit
IDIBELL
Antonella Consiglio
ORCID ID: 0000-0002-3988-6254
Tel +34 934039842
https://consigliolaboratory.com/
Principal Investigator: • Antonella Consiglio
Senior Post-Doc: • Meritxell Pons Espinal
Post-Doc: • Carles Calatayud
Research Assistant: • Irene Fernández Carasa
Postgraduate fellow: • Isabel Turpin
PhD Student: • Giulia Carola • Lucas Blasco • Agell Silvia • Beltramone • Alba Tristan Noguero
Current
Research
The primary focus of our lab is to examine the underlying molecular mechanisms that drive the development of Parkinson’s disease (PD). Consiglio lab uses stem cells, mainly patient-derived induced pluripotent stem cells (iPSCs) derived from idiopathic and familial PD skin cells as well as healthy age-matched controls, aiming to decipher the mechanisms that lead to PD brain cell dysfunction and degeneration (Embo Mol.Med. 2012, Nat. Neuroscience 2013, Embo Mol. Med.2015).
Our lab derives dopaminergic neurons, as well as glial cells (astrocytes & microglia) to fully investigate pathological mechanisms that occur during PD. We have successfully used iPSC-derived cells as a platform to test possible PD therapies and have developed a co-culturing system to really have a birds-eye view into a PD patients’ brain. We also use gene editing techniques to further decipher PD pathology.
Recently novel tools have been developed in the laboratory, which will facilitate the study of disease-modifying, restorative and regenerative strategies for PD.
Selected
Publications
Calatayud, C.; Carola, G.; Fernández-Carasa, I.; Valtorta, M.; Jiménez-Delgado, S.; Díaz, M.; Soriano-Fradera, J.; Cappelletti, G.; García-Sancho, J.; Raya, Á.; Consiglio, A. CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons. Scientific Reports. 9(1):6811, 2019. doi.org/10.1038/s41598-019-43080-2
Requena, J.; Alvarez-Palomo, A. B.; Codina-Pascual, M.; Delgado-Morales, R.; Moran, S.; Esteller, M.; Sal, M.; Juan, M.; Boronat-Barado A.; Consiglio, A.; Bogle, O. A.; Wolvetang, E.; Ovchinnikov, D.; Alvarez, I.; Jaraquemada, D.; Mezquita-Pla, J.; Oliva, R.; Edel, M. J. Global Proteomic and Methylome Analysis in Human Induced Pluripotent Stem Cells Reveals Overexpression of a Human TLR3 Affecting Proper Innate Immune Response Signaling. Stem Cells. 37(4):476-488, 2019. doi.org/10.1002/stem.2966.
di Domenico, A.; Carola, G.; Calatayud, C.; Pons-Espinal, M.; Muñoz, J. P.; Richaud-Patin, Y.; Fernandez-Carasa, I.; Gut, M.; Faella, A.; Parameswaran, J.; Soriano, J.; Ferrer, I.; Tolosa, E.; Zorzano, A.; Cuervo, A. M.; Raya, A.; Consiglio, A. Patient-Specific iPSC-Derived Astrocytes Contribute to Non-Cell-Autonomous Neurodegeneration in Parkinson’s Disease. Stem Cell Reports. 12(2), 213-229, 2019. doi.org/10.1016/j.stemcr.2018.12.011
Bayó-Puxan, N.; Terrasso, A. P.; Creyssels, S.; Simão, D.; Begon-Pescia, C.; Lavigne, M.; Salinas, S.; Bernex, F.; Bosch, A.; Kalatzis, V.; Levade, T.; Cuervo, A. M.; Lory, P.; Consiglio, A.; Brito, C.; Kremer, E. J. Lysosomal and network alterations in human mucopolysaccharidosis type VII iPSC-derived neurons. Scientific Reports. 8(1):16644, 2018. doi.org/10.1038/s41598-018-34523-3
Suh, H.; Zhou, Q. G.; Fernandez-Carasa, I.; Clemenson, G. D. Jr.; Pons-Espinal, M.; Ro, E. J.; Marti, M.; Raya, A.; Gage, F. H.; Consiglio, A. Long-Term Labeling of Hippocampal Neural Stem Cells by a Lentiviral Vector. Frontiers in Molecular Neuroscience. 11:415, 2018. doi.org/10.3389/fnmol.2018.00415
