Stem Cells, Development and Disease Lab

Department of Cell Biology, Physiology and Immunology

Faculty of Biology, UB

Ana Sevilla Hernández
ORCID ID: 0000-0002-9251-4759

https://sites.google.com/view/sevillalab/home

Principal investigator: • Ana Sevilla Hernández
Research Technician: • Margarita Garcia • Verónica González
PhD student: • Ylenia dellarocca • Carlos Mateos Sanchez
Student: • Maria Manterola Larrañaga

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Current
Research

The Sevilla lab uses stem cells, mainly patient-derived induced pluripotent stem cells (iPSCs), to decipher the regulatory mechanisms that govern pluripotency and neuronal development. As molecular tools, we also use CRISPR/Cas9 gene editing techniques to further understand specific pathologies.

Our lab derives brain organoids to investigate pathological mechanisms where neuroinflamation plays a key role. The primary focus of our lab is to examine the underlying molecular mechanisms that drive neuronal development to better understand neuroinflamatory mechanisms.

Selected
Publications

Pedrosa L.; Bedia C.; Diao D.; Mosteiro A.; Ferrés A.; Stanzani E.; Martínez-Soler F.; Tortosa A.; Pineda E.; Aldecoa I.; Centellas M.; Muñoz-Tudurí M.; Sevilla A.; Sierra À.; González Sánchez J. J. Preclinical Studies with Glioblastoma Brain Organoid Co-Cultures Show Efficient 5-ALA Photodynamic Therapy. Cells. 12(8):1125, 2023. doi.org/10.3390/cells12081125

Petazzi P.; Miquel-Serra L.; Huertas S.; González C.; Boto N.; Muñiz-Diaz E.; Menéndez P.; Sevilla A.; Nogués N. ABO gene editing for the conversion of blood type A to universal type O in Rhnull donor-derived human-induced pluripotent stem cells. Clinical and Translational Medicine. 2022 12(10):e1063, 2022. doi.org/10.1002/ctm2.1063

Mazloom A. R.; Xu H.; Reig-Palou J.; Vasileva A.; Román A. C.; Mulero-Navarro S.; Lemischka I. R.; Sevilla A. Esrrb Regulates Specific Feed-Forward Loops to Transit From Pluripotency Into Early Stages of Differentiation.  Frontiers in Cell and Developmental Biology. 10:820255, 2022. doi.org/10.3389/fcell.2022.820255

Sevilla A.; Papatsenko D.; Mazloom A. R.; Xu H.; Vasileva A.; Unwin R. D.; LeRoy G.; Chen E, Y.; Garrett-Bakelman F. E.; Lee D. F.; Trinite B.; Webb R. L.; Wang Z.; Su J.; Gingold J.; Melnick A.; Garcia B. A.; Whetton A. D.; MacArthur B. D.; Ma’ayan A.; Lemischka IR. An Esrrb and Nanog Cell Fate Regulatory Module Controlled by Feed Forward Loop Interactions. Frontiers in Cell and Developmental Biology. 9:630067, 2021. doi.org/10.3389/fcell.2021.630067

Petazzi P.; Menéndez P.; Sevilla A. CRISPR/Cas9-Mediated Gene Knockout and Knockin Human iPSCs. Methods in Molecular Biology. 2020. doi.org/10.1007/7651_2020_337

Selected
Publications