Antonio Fernandez, Nicola Kielland, Ashraff Makda, Neil O. Carragher, M. Carmen González-García, Laura Espinar-Barranco, Juan A. González-Vera, Angel Orte, Rodolfo Lavilla and Marc Vendrell
DOI: 10.1039/D2CB00168C
We report a platform combining multicomponent reaction synthesis and automated cell-based screening to develop biocompatible NIR-BODIPY fluorophores. From a library of over 60 fluorophores, we optimised compound NIRBD-62c as a multimodal probe with suitable properties for STED super-resolution and fluorescence lifetime imaging. Furthermore, we employed NIRBD-62c for imaging trafficking inside cells and to examine how pharmacological inhibitors can alter the vesicular traffic between intracellular compartments and the plasma membrane.
