Molecular basis of membrane traffic in mammalian cells - PI: Carles Enrich Bastús

Presentation

In the last years, our research group has focused on fundamental aspects of the traffic of proteins, metabolism and signaling at the cellular level, and the in vivo consequences in animal models. Intracellular compartments and organelles maintain interactions that are important for regulating metabolic and cellular processes. In this sense, it has been shown that the AMPK microtubule-dynamics regulation allows the interaction of mitochondria and lipid droplets to ensure energy requirements when fasting. On the other hand, it has been demonstrated that the AnxA6 located in the endocytic compartment regulates intracellular cholesterol levels and its cellular homeostasis. In situations such as NPC1 disease, or experimentally after overexpressing AnxA6, cholesterol accumulates in endosomes. This induces mislocalization of a group of SNARE proteins, interfering the recycling of integrins, and consequently generating a reduction in cell migration and invasion. We have also analyzed the dynamic’s association of small GTPases, such as KRas and Rac1, with endosome membranes, and the results have highlighted the role of these proteins in the endocytic compartment signaling to regulate cellular processes such as cell migration.

Currently, our research is organized into 3 main interdisciplinary lines to understand the molecular mechanisms that translate cell surface originated signals into metabolic and functional changes within the cells. Our research groups are:

  1. Endocytosis and intracellular trafficking of proteins and cholesterol (IP: Carles Enrich)
  2. Lipid trafficking and metabolic disease (IP: Albert Pol)
  3. Signalling, cytoskeleton and cellular migration (IP: Francesc Tebar)

Below are the names and email addresses of the members of each of the teams that make up the different lines of research of the research group:
 

  • Team: Endocytosis and intracellular trafficking of proteins and cholesterol

Principal Investigator:

Carles Enrich Bastús (enrich@ub.edu)
Casanova 143
3a Planta Facultat de Medicina ala est
Tel.: 34-934021908
Fax. 34-934021907

Postdoctoral researchers:

Albert Luis Lu Lopez (alulopez@stanford.edu)              
Carles Rentero Alfonso (carles.rentero@ub.edu)
Teresa Royo Bargués (teresaroyo@ub.edu)

 

  • Team: Lipid trafficking and metabolic disease

Principal Investigator:

Albert Pol Sorolla (apols@ub.edu)
Casanova 143
1ª planta Facultat de Medicina ala sud
CELLEX 1 Bloc A
Tel. 93 2275400 ext. 3358
Fax. 93 4021907

Postdoctoral researchers:

Marta Bosch Rodríguez (martabosch@ub.edu)
Albert Gubern Burset (albertgubern@gmail.com)

Predoctoral researchers:

Frederic Morales Paytubí (moralesfredericpaytuvi@gmail.com)

Laboratory technician:

Alba Fajardo González (albafajardogonzalez@gmail.com)

 

  • Team: Signalling, cytoskeleton and cellular migration

Principal Investigator:

Francesc Tebar Ramon (tebar@ub.edu)
Casanova 143
1ª planta Facultat de Medicina ala sud
CELLEX 1 Bloc A
Tel. 93 2275400 ext. 3358
Fax. 93 4021907

Postdoctoral researchers:

Maria Calvo Adamúz (mariacalvo@ub.edu)

Predoctoral researchers:

Albert Chavero Pieres (albertchavero@gmail.com)

Laboratory location:

Lab. 1B
C/Casanova 143, 1ª planta Facultat de Medicina ala sud
Recepció CELLEX 1 Bloc A
Tel. 93 2275400 ext. 3358
Fax. 93 4021907

  • Team: Endocytosis and intracellular trafficking of proteins and cholesterol (IP: Carles Enrich)

Our group is interested on the molecular mechanisms of membrane traffic in mammalian cells. This basic research is important for understanding a number of diseases including cancer, diabetes, and heart disease or liver disorders. We study the regulation of a variety of aspects of protein and cholesterol trafficking and signal transduction in the endocytic compartment. We focus on determining the role of annexin A6 in signalling, membrane trafficking and liver regeneration and glucose metabolism.

 

  • Team: Lipid trafficking and metabolic disease (IP: Albert Pol)

Lipid droplets are organelles that collect, store and supply lipids in all eukaryotic cells. These organelles are also cellular hallmarks of prevalent diseases ranging from obesity to cancer. The cell biology of lipid droplets and its role in pathogenesis are still unclear. Our group is interested in the mechanisms of lipid droplet biogenesis and consumption. In this sense, the aim of our project is to characterize - in health and in disease - the cellular processes that are regulated or altered due to changes of the lipid fluxes existing in our cells. For instance, cholesterol accumulation promotes mitochondrial dysfunction and oxidative damage underlying in the pathogenesis of steatohepatitis, neurodegeneration and atherosclerosis. In this context, we are especially interested in how caveolin-1 regulates cholesterol trafficking and storage.

 

  • Team: Signalling, cytoskeleton and cellular migration (IP: Francesc Tebar)

Our group is studying the signaling and trafficking of the epidermal growth factor receptor (EGFR) and the small GTPases KRas and Rac1. The signaling elicited by these proteins controls various important cellular processes such as proliferation, cell migration and mobility. EGFR signaling is initiated at the plasma membrane and remains in the endosomes, these signaling platforms can move to different cellular locations, thereby restricting spatial signaling and controlling different processes in the cell. Accordingly, many signaling proteins of EGFR have been detected in endosomes, among them KRas and Rac1. One of the objectives of the group is to study the involvement of the endocytic compartment on EGFR, KRas and Rac1 functionality.

  • Zonal distribution of the SARS CoV-2 receptor ACE2 and its partner TMPRSS2 in chronic liver disease

Principal Investigator: José Carlos Fernández-Checa
Team researchers: Carmen García Ruiz and Carlos Enrich
Funding Entity: scholarships of the Asociación Española para el Estudio del Hígado (AEEH): COVID-LIVER (2020). HCB/2020/1172.
Period: 2020- . . .

  • Iberoamerican Consortium for the Study of Liver Cirrhosis: A new avenue for therapeutics

Principal Investigator: Xavier Cubero (Coordinator, Madrid).
Team researchers: C Enrich (Barcelona), H Nakaya (Sao Paulo), NR Vargas (Buenos Aires) and RU Macías-Rodriguez (México)
Funding Entity: Unión Iberoamericana de Universidades. Financiación de proyectos de Investigación Interuniversitaria.
Period: 2019-2020

  • Redes temáticas de investigación: Enfermedades Metabólicas y Cancer. RED2018-102799-T.

Principal Investigator: José Carlos Fernández-Checa
Team researchers: Total 9 groups
Funding Entity: Ministerio de Ciencia, Innovación y Universidades (2019)
Period: 2019 -

  • Lipids and Synaptic Signaling in Health and Disease

Principal investigator: Alejandra Alvarez (Universidad de Chile)
Team researchers: Silvana Zanlungo, Carlos Enrich, Mónica Vazquez and Francisco Muñoz
Funding Entity: Concurso de apoyo a la formación de Redes Internacionales entre Centros de Investigación. CONICYT 2018
Period: 2018 -
Amount: 15.900.000 CLP$.

  • Contribución del compartimiento endocítico a la migración celular: Regulación por colesterol, annexin A6 y Rac1

Principal investigators: Carles Enrich and Francesc Tebar
Funding Entity: Ministerio de Economía y Competitividad. BFU2015-66785-P
Period: 01/01/2016-31/12/2019

  • Organización compartimental y transporte

Principal investigators: Carles Enrich and Albert Pol (IP Coordinator: Vivek Malhotra)
Entitat Finançadora: Redes de Excelencia. REDES CONSOLIDER. 2016. Ministerio de Economía y Competitividad. BFU2016-81912-REDC (2017-2019)
Period: 01/01/2017-31/12/2019

  • Mammalian lipid droplets: a central role in the organismal antibacterial response?

Principal investigator: Albert Pol
Funding Entity: Human Frontier Science Program (HFSP). RGP0020/2015
Period: 01/06/2015-31/05/2018

  • Nuevas funciones de ACSL3, CAV1 y colesterol en el retículo endoplásmico: sensores homeostáticos que determinan la supervivencia o la muerte de las células.

Principal investigator: Albert Pol
Funding Entity: Ministerio de Economia y Competitividad. BFU2015-66401-R
Period: 01/01/2016-31/12/2018

  • Title: Lipid Droplet Overloading Promotes Pathogenesis and Progression of Type 2 Diabetes: Identifying New Therapeutic Targets and Applying Upgraded Therapies. 31/U/2016.

Principal investigator: Albert Pol
Funding Entity: Fundació Marató de TV3.
Period: 01/01/2017-31/12/2019

For more information for PI publications click in the link:
ORCID: https://orcid.org/0000-0003-0382-2993

Anna Alvarez-Guaita, Patricia Blanco-Muñoz, Elsa Meneses-Salas, Mohamed Wahba, Abigail H. Pollock, Jaimy Jose, Mercedes Casado, Marta Bosch, Rafael Artuch, Katharina Gaus, Albert Lu, Albert Pol, Francesc Tebar, Stephen E. Moss, Thomas Grewal, Carlos Enrich** Carles Rentero. Annexin A6 is critical to maintain glucose homeostasis and survival during liver regeneration in mice. Hepatology (2020) 72 (6), 2149-2164. doi: 10.1002/hep.31232. PMID: 32170749.

Elsa Meneses-Salas, Ana García-Melero, Patricia Blanco-Muñoz, Jaimy Jose, Marie-Sophie Brenner, Albert Lu, Francesc Tebar, Thomas Grewal, Carles Rentero and Carlos Enrich*. Selective degradation permits a feedback loop controlling Annexin A6 and cholesterol levels in endolysosomes of NPC1 mutant cells. Cells (2020) May 7;9(5):E1152. doi: 10.3390/cells9051152. PMID: 32392809.

Francesc Tebar, Albert Chavero, Carles Rentero, Albert Lu, Neus Agell, Carlos Enrich and Thomas Grewal. Pleiotropic roles of calmodulin in the regulation of KRas and Rac1 GTPases: functional diversity in health and disease. International Journal of Molecular Sciences (2020) 21 (10): 3680 doi: 10.3390/ijms21103680. PMID: 32456244.

Marta Bosch, Miguel Sánchez-Álvarez, Alba Fajardo, Ronan Kapetanovic, Bernhard Steiner, Filipe Dutra, Luciana Moreira, Juan Antonio López, Rocio Campo, Montserrat Marí, Frederic Morales-Paytuví, Olivia Tort, Albert Gubern, Rachel M. Templin, James E. B. Curson, Nick Martel, Cristina Català, Francisco Lozano, Francesc Tebar, Carlos Enrich, Jesús Vázquez, Miguel A. Del Pozo, Matthew J. Sweet, Patricia T. Bozza, Steven P. Gross, Robert G. Parton and Albert Pol. Mammalian lipid droplets are innate immune hubs integrating cell metabolism and host defense. Science (2020) Oct 16;370(6514):eaay8085. 370.doi:10.1126/science.eaay8085. PMID: 33060333.

Elsa Meneses-Salas, Marta García-Forn, Marta Bosch, Jaimy Jose, Mohamed Wahba, Albert Pol, Francesc Tebar, Albert Lu, Andrés D. Klein, Silvana Zanlungo, Esther Pérez-Navarro, Thomas Grewal, Carlos Enrich** and Carles Rentero. Lack of Annexin A6 exacerbates liver dysfunction and reduced lifespan of NPC1-deficient mice. American Journal of Pathology (2020) S0002-9440 (20) 30564-2. doi.org/10.1016/j.ajpath.2020.12.009. PMID: 33345999.

Faten Abdelli, Karim Jellali, Estefanía Anguita, María González-Muñoz, Eduardo Villalobo, Ivan Madroñal, Juan Alcalde, Mamdouh Ben Ali, Jihene Elloumi-Mseddi, Ikram Jemel, Francesc Tebar, Carlos Enrich, Sami Aifa and Antonio Villalobo. The role of the calmodulin-binding and calmodulin-like domains of the epidermal growth factor receptor in tyrosine kinase activation. Journal of Cellular Physiology (2020) doi: 10.1002/jcp.30205. PMID: 33305427.

Hoque M, Elmaghrabi YA, Köse M, Meneses-Salas E, Blanco-Muñoz P, Beevi SS, Jose J, Conway JR, Swarbrick A, Timpson P, Tebar F, Enrich C, Rentero C, Grewal T. Annexin A6 improves anti-migratory properties of tyrosine kinase inhibitors in EGFR overexpressing human squamous epithelial cells. Febs J. 2019 Dec 23. doi: 10.1111/ febs. 15186. [Epub ahead of print]

Enrich C, Rentero C, Grewal T, Futter CE, Eden ER. Cholesterol overload: contact sites to the rescue! Contact (Thousand Oaks). doi: 10.1177/ 2515256419893507. Author manuscript; available in PMC 2019 Dec 19. PMID: 31858076

Meneses-Salas E, García-Melero A, Kanerva K, Blanco-Muñoz P, Morales-Paytuvi F, Bonjoch J, Casas J, Egert A, Beevi SS, Jose J, Llorente-Cortés V, Rye K-A, Heeren J, Lu A, Pol A, Tebar F, Ikonen E, Grewal T, Enrich C**, Rentero C. Annexin A6 modulates TBC1D15/Rab7/StARD3 axis to control endosomal cholesterol export in NPC1 cells. Cell Mol Life Sci. 2019 Oct 29. doi: 10.1007/ s00018-019-03330-y. [Epub ahead of print]. PMID: 31664461

Grewal T, Enrich C, Rentero C, Buechler C. Annexins in adipose tissue: Novel players in obesity. Int J Mol Sci. 2019 Jul 13; 20(14). pii: E3449. doi: 10.3390 /ijms 20143449. PMID: 31337068

Solsona-Vilarrasa E, Fucho R, Torres S, Nuñez S, Nuño-Lámbarri N, Enrich C, García-Ruiz C, Fernández-Checa JC. Cholesterol enrichment in liver mitochondria impairs oxidative phosphorylation and disrupts the assembly of respiratory supercomplexes. Redox Biol. 2019 Jun; 24:101214. doi: 10.1016 /j.redox. 2019.101214. Epub 2019 May 9. PMID: 31108462

Cairns R, Fischer AW, Blanco-Munoz P, Alvarez-Guaita A, Meneses-Salas E, Egert A, Buechler C, Hoy AJ, Heeren J, Enrich C, Rentero C, Grewal T. Altered hepatic glucose homeostasis in AnxA6-KO mice fed a high-fat diet. PLoS One. 2018 Aug 15;13(8): e0201310. doi: 10.1371 /journal.pone. 0201310. eCollection 2018. PMID: 30110341

Tebar F, Enrich C, Rentero C, Grewal T. GTPases Rac1 and Ras Signaling from Endosomes. Prog Mol Subcell Biol. 2018;57:65-105. doi: 10.1007/978-3-319- 96704-2_3. Review. PMID: 30097772

Rentero C, Blanco-Muñoz P, Meneses-Salas E, Grewal T, Enrich C. Annexins-Coordinators of Cholesterol Homeostasis in Endocytic Pathways. Int J Mol Sci. 2018 May 12;19(5). pii: E1444. doi: 10.3390/ ijms19051444. Review. PMID: 29757220

Torres S, Balboa E, Zanlungo S, Enrich C, Garcia-Ruiz C, Fernandez-Checa JC. Lysosomal and Mitochondrial Liaisons in Niemann-Pick Disease. Front Physiol. 2017 Nov 30;8:982. doi: 10.3389/ fphys.2017. 00982. eCollection 2017. Review. PMID: 29249985

Soriano-Castell D, Chavero A, Rentero C, Bosch M, Vidal-Quadras M, Pol A, Enrich C, Tebar F. ROCK1 is a novel Rac1 effector to regulate tubular endocytic membrane formation during clathrin-independent endocytosis. Sci Rep 2017; Jul 31;7(1):6866. doi: 10.1038/ s41598-017- 07130-x.

Cairns R, Alvarez-Guaita A, Martínez-Saludes I, Wason SJ, Hanh J, Nagarajan SR, Hosseini-Beheshti E, Monastyrskaya K, Hoy AJ, Buechler C, Enrich C, Rentero C, Grewal T. Role of hepatic Annexin A6 in fatty acid-induced lipid droplet formation. Exp Cell Res 2017; Sep 15;358(2): 397-410. doi: 10.1016/ j.yexcr.2017.07.015.

Grewal T, Hoque M, Conway JRW, Reverter M, Wahba M, Beevi SS, Timpson P, Enrich C, Rentero C. Annexin A6-A multifunctional scaffold in cell motility. Cell Adh Migr 2017; May 4;11(3):288-304. doi: 10.1080/ 19336918. 2016. 1268318.

Fleta-Soriano E, Smutná K, Martínez JP, Lorca Oró C, Sadiq SK, Mirambeau G, Lopez-Iglesias C, Bosch M, Pol A, Brönstrup M, Diez J, Meyerhans A. The Myxobacterial Metabolite Soraphen A Inhibits HIV-1 by Reducing Virus Production and Altering Virion Composition. Antimicrob Agents Chemother. 2017; Jul 25;61(8). pii: e00739-17. doi: 10.1128/AAC.00739-17.

Enrich C, Rentero C, Grewal T. Annexin A6 in the liver: From the endocytic compartment to cellular physiology. Biochim Biophys Acta 2017; Jun; 1864(6): 933-946. doi: 10.1016/ j.bbamcr. 2016.10.017.

Torres S, Matías N, Baulies A, Nuñez S, Alarcon-Vila C, Martinez L, Nuño N, Fernandez A, Caballeria J, Levade T, Gonzalez-Franquesa A, Garcia-Rovés P, Balboa E, Zanlungo S, Fabrías G, Casas J, Enrich C, Garcia-Ruiz C, Fernández-Checa JC. Mitochondrial GSH replenishment as a potential therapeutic approach for Niemann Pick type C disease. Redox Biol 2017; Apr; 11:60-72. doi: 10.1016/ j.redox.2016.11.010.

Krautbauer S, Haberl EM, Eisinger K, Pohl R, Rein-Fischboeck L, Rentero C, Alvarez-Guaita A, Enrich C, Grewal T, Buechler C, Neumeier M. Annexin A6 regulates adipocyte lipid storage and adiponectin release. Mol Cell Endocrinol 2017; Jan 5;439: 419-430. doi: 10.1016/ j.mce. 2016.09.033.

Villarroya-Beltri C, Baixauli F, Mittelbrunn M, Fernández-Delgado I, Torralba D, Moreno-Gonzalo O, Baldanta S, Enrich C, Guerra S, Sánchez-Madrid F. ISGylation controls exosome secretion by promoting lysosomal degradation of MVB proteins. Nat Commun 2016; 7:13588. doi: 10.1038/ ncomms13588.

Sala-Vila A, Navarro-Lérida I, Bosch M, Calvo C, López JA, Calvo E, Ferguson C, Balsinde J, Enríquez JA, Giacomello M, Scorrano L, Parton RG, Vázquez J, Pol A, del Pozo MA. Interplay between hepatic mitochondria-associated membranes, lipid metabolism and caveolin-1 in mice. Sci Rep 2016; 6:27351. doi: 10.1038/ srep27351.

Cornely R, Pollock AH, Rentero C, Norris SE, Alvarez-Guaita A, Grewal T, Mitchell T, Enrich C, Moss SE, Parton RG, Rossy J, Gaus K. Annexin A6 regulates interleukin-2-mediated T-cell proliferation. Immunol Cell Biol 2016; 94(6): 543-53. doi: 10.1038/ icb.2016.15.

García-Melero A, Reverter M, Hoque M, Meneses-Salas E, Koese M, Conway J R, Johnsen C H, Alvarez-Guaita A, Morales-Paytuvi F, Elmaghrabi Y A, Pol A, Tebar F, Murray R Z, Timpson P, Enrich C, Grewal T, Rentero C. Annexin A6 and late endosomal cholesterol modulates integrin recycling and cell migration. J Biol Chem 2016; 291(3): 1320-1335. doi: 10.1074/ jbc.M115.683557.

Bosch M, Fajardo A, Alcalá-Vida R, Fernández-Vidal A, Tebar F, Enrich C, Cardellach F, Pérez E, Pol A. Hepatic Primary and Secondary Cholesterol Deposition and Damage in Niemann-Pick Disease. Am J Pathol. 2016; 186 (3):517-23. doi: 10.1016/j.ajpath.2015.12.002.

Tran J, Magenau A, Rodriguez M, Rentero C, Royo T, Enrich C, Thomas SR, Grewal T, Gaus K. Activation of Endothelial Nitric Oxide (eNOS) Occurs through Different Membrane Domains in Endothelial Cells. PLoS One. 2016; 11(3): e0151556. doi: 10.1371/ journal.pone. 0151556.

Grewal T, Wason SJ, Enrich C, Rentero C. Annexins - insights from knockout mice. Biol Chem 2016; 397(10): 1031-53. doi: 10.1515/ hsz- 2016-0168.

Alvarez-Guaita A, Vila de Muga S, Owen DM , Williamson D, Magenau A, Garcia- Melero A, Reverter M, Hoque M, Cairns R, Cornely R, Tebar F, Grewal T, Gaus K, Ayala- Sanmartin J, Enrich C, Rentero C. Evidence for annexin A6-dependent plasma membrane remodelling of lipid domains. Br J Pharmacol. 2015; 172, 1677-1690.  doi: 10.1111/ bph.13022.

Herms A, Bosch M, Reddy B, Schieber N, Fajardo A, Rupérez C, Fernández-Vidal A, Rentero C, Tebar F, Enrich C, Parton RG, Gross SP, Pol A. AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation. Nat Commun. 2015; 6:7176. doi: 10.1038/ ncomms8176.

Reales E, Bernabé-Rubio M, Casares- Arias J, Rentero C, Enrich C, Andrés G, Alonso MA. The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation. J Cell Sci 2015; 128, 2261-2270. doi: 10.1242/ jcs.164970.

Enrich C, Rentero C, Hierro A, Grewal T. Role of cholesterol in SNARE-mediated trafficking on intracellular membranes. J Cell Sci 2015; 128, 1071-1081. doi: 10.1242/ jcs.164459.

Hoque H, Rentero C, Conway JR, Timpson P, Murray RZ, Enrich C, Grewal T. The cross-talk of LDL-cholesterol with cell motility: insights from the Niemann Pick Type C1 mutation and altered integrin trafficking. Cell Adh Migr 2015; 9, 384-391. doi: 10.1080/ 19336918. 2015.1019996.

Gelabert-Baldrich M, Soriano-Castell D, Calvo M, Lu A, Vina-Vilaseca A, Rentero C, Pol A, Grinstein S, Enrich C, Tebar F. Dynamics of KRas on endosomes: involvement of acidic phospholipids in its association. FASEB J 2014;28: 3023-3037. doi: 10.1096/ fj.13-241158.

Reverter M, Rentero C, Garcia-Melero A, Hoque M, Vila de Muga S, Alvarez-Guaita A, Conway JR, Wood P, Cairns R, Lykopoulou L, Grinberg D, Vilageliu L, Bosch M, Heeren J, Blasi J, Timpson P, Pol A, Tebar F, Murray RZ, Grewal T, Enrich C. Cholesterol regulates Syntaxin 6 trafficking at trans-Golgi network endosomal boundaries. Cell Rep 2014; 7:883-897.  doi: 10.1016/ j.celrep.2014.03.043

Rodrigo-Torres D, Affo S, Coll M, Morales-Ibanez O, Millan C, Blaya D, Alvarez-Guaita A, Rentero C, Lozano JJ, Maestro MA, Solar M, Arroyo V, Caballeria J, van Grunsven LA, Enrich C, Gines P, Bataller R, Sancho-Bru P. The biliary epithelium gives rise to liver progenitor cells. Hepatology 2014;60: 1367-1377. doi: 10.1002/ hep.27078.

Pol A, Gross SP, Parton RG. Biogenesis of the multifunctional lipid droplet: lipids, proteins, and sites. J Cell Biol 2014; 204:635-646. doi: 10.1083/ jcb.201311051.

Hoque M, Rentero C, Cairns R, Tebar F, Enrich C, Grewal T. Annexins - scaffolds modulating PKC localization and signaling. Cell Signal 2014; 26:1213-1225. doi: 10.1016/ j.cellsig.2014.02.012.

Tebar F, Gelabert-Baldrich M, Hoque M, Cairns R, Rentero C, Pol A, Grewal T, Enrich C. Annexins and endosomal signaling. Methods Enzymol 2014; 535:55-74. doi: 10.1016/ B978-0-12-397925- 4.00004-3.

Soler M, González-Bártulos M, Soriano-Castell D, Ribas X, Costas M, Tebar F, Massaguer A, Feliu L, Planas M. Identification of BP16 as a non-toxic cell-penetrating peptide with highly efficient drug delivery properties. Org Biomol Chem. 2014; Mar 14;12(10): 1652-63. doi: 10.1039/ c3ob42422g.

Kassan A, Herms A, Fernández-Vidal A, Bosch M, Schieber NL, Reddy BJ, Fajardo A, Gelabert-Baldrich M, Tebar F, Enrich C, Gross SP, Parton RG, Pol A. Acyl-CoA synthetase 3 promotes lipid droplet biogenesis in ER microdomains. J Cell Biol. 2013; Dec 23;203(6): 985-1001. doi: 10.1083/ jcb.201305142.

Herms A, Bosch M, Ariotti N, Reddy BJ, Fajardo A, Fernández-Vidal A, Alvarez-Guaita A, Fernández-Rojo MA, Rentero C, Tebar F, Enrich C, Geli MI, Parton RG, Gross SP, Pol A. Cell-to-cell heterogeneity in lipid droplets suggests a mechanism to reduce lipotoxicity. Curr Biol. 2013 Aug 5;23(15): 1489-96. doi: 10.1016/ j.cub.2013. 06.032.

Barceló C, Paco N, Beckett AJ, Alvarez-Moya B, Garrido E, Gelabert M, Tebar F, Jaumot M, Prior I, Agell N.Oncogenic K-Ras segregates at spatially distinct plasma membrane signaling platforms according to its phosphorylation status. J Cell Sci. 2013 Aug 13. doi: 10.1242/ jcs.123737.

Panosa C, Tebar F, Ferrer-Batallé M, Fonge H, Seno M, Reilly RM, Massaguer A, De Llorens R. Development of an epidermal growth factor derivative with EGFR blocking activity. PLoS One. 2013 Jul 30;8(7): e69325. doi: 10.1371/ journal.pone. 0069325.

Koese M, Rentero C, Kota BP, Hoque M, Cairns R, Wood P, Vilà de Muga S, Reverter M, Alvarez-Guaita A, Monastyrskaya K, Hughes WE, Swarbrick A, Tebar F, Daly RJ, Enrich C, Grewal T. Annexin A6 is a scaffold for PKCα to promote EGFR inactivation. Oncogene. 2013 Jun 6;32(23): 2858-72.

Mulay V, Wood P, Manetsch M, Darabi M, Cairns R, Hoque M, Chan KC, Reverter M, Alvarez-Guaita A, Rye KA, Rentero C, Heeren J, Enrich C, Grewal T. Inhibition of mitogen-activated protein kinase Erk1/2 promotes protein degradation of ATP binding cassette transporters A1 and G1 in CHO and HuH7 cells. PLoS One 2013; Apr 25;8(4): e62667. doi: 10.1371/ journal.pone. 0062667.

Anand P, Cermelli S, Li Z, Kassan A, Bosch M, Sigua R, Huang L, Ouellette AJ, Pol A, Welte MA, Gross SP. A novel role for lipid droplets in the organismal antibacterial response. Elife. 2012;1: e00003.  doi: 10.7554/ eLife.00003.

Duran JM, Campelo F, van Galen J, Sachsenheimer T, Sot J, Egorov MV, Rentero C, Enrich C, Polishchuk RS, Goñi FM, Brügger B, Wieland F, Malhotra V. Sphingomyelin organization is required for vesicle biogenesis at the Golgi complex. EMBO J. 2012 Dec 12;31(24): 4535-46. doi: 10.1038/ emboj.2012.317.

Navarro-Lérida I, Sánchez-Perales S, Calvo M, Rentero C, Zheng Y, Enrich C, Del Pozo MA. A palmitoylation switch mechanism regulates Rac1 function and membrane organization. EMBO J. 2012 Feb 1;31(3): 534-51. doi: 10.1038/ emboj.2011.446.

Fernández-Rojo MA, Restall C, Ferguson C, Martel N, Martin S, Bosch M, Kassan A, Leong GM, Martin SD, McGee SL, Muscat GE, Anderson RL, Enrich C, Pol A, Parton RG. Caveolin-1 orchestrates the balance between glucose and lipid-dependent energy metabolism: implications for liver regeneration. Hepatology. 2012 May; 55(5): 1574-84. doi: 10.1002/ hep.24810.

Mulay V, Wood P, Rentero C, Enrich C, Grewal T. Signal transduction pathways provide opportunities to enhance HDL and apoAI-dependent reverse cholesterol transport. Curr Pharm Biotechnol 2012; Feb;13(2): 352-64.

Owen DM, Rentero C, Magenau A, Abu-Siniyeh A, Gaus K. Quantitative imaging of membrane lipid order in cells and organisms. Nat Protoc 2011; Dec 8;7(1): 24-35.  doi: 10.1038/ nprot.2011.419.

Reverter M, Rentero C, de Muga SV, Alvarez-Guaita A, Mulay V, Cairns R, Wood P, Monastyrskaya K, Pol A, Tebar F, Blasi J, Grewal T, Enrich C. Cholesterol transport from late endosomes to the Golgi regulates t-SNARE trafficking, assembly, and function. Mol Biol Cell. 2011 Nov;22(21): 4108-23. doi: 10.1091/ mbc.E11-04-0332.

Bosch M, Marí M, Gross SP, Fernández-Checa JC, Pol A. Mitochondrial cholesterol: a connection between caveolin, metabolism, and disease. Traffic. 2011 Nov;12(11): 1483-9. doi: 10.1111/ j.1600-0854. 2011.01259.x.

Cornely R, Rentero C, Enrich C, Grewal T, Gaus K. Annexin A6 is an organizer of membrane microdomains to regulate receptor localization and signalling. IUBMB Life. 2011 Nov;63(11): 1009-17. doi: 10.1002/ iub.540.

Vidal-Quadras M, Gelabert-Baldrich M, Soriano-Castell D, Lladó A, Rentero C, Calvo M, Pol A, Enrich C, Tebar F. Rac1 and calmodulin interactions modulate dynamics of ARF6-dependent endocytosis. Traffic. 2011 Dec;12(12): 1879-96.  doi: 10.1111/ j.1600-0854. 2011.01274.x. 

Astudillo AM, Pérez-Chacón G, Meana C, Balgoma D, Pol A, Del Pozo MA, Balboa MA, Balsinde J. Altered arachidonate distribution in macrophages from caveolin-1 null mice leading to reduced eicosanoid synthesis. J Biol Chem. 2011 Oct 7; 286(40): 35299-307. doi: 10.1074/ jbc.M111.277137.

Wood P, Mulay V, Darabi M, Chan KC, Heeren J, Pol A, Lambert G, Rye KA, Enrich C, Grewal T. Ras/mitogen-activated protein kinase (MAPK) signaling modulates protein stability and cell surface expression of scavenger receptor SR-BI. J Biol Chem 2011; Jul 1;286(26): 23077-92. doi: 10.1074/ jbc.M111.236398.

Grewal T, Koese M, Tebar F, Enrich C. Differential Regulation of RasGAPs in Cancer. Genes Cancer. 2011 Mar;2(3): 288-97. doi: 10.1177/ 1947601911407330.

Alvarez-Moya B, Barceló C, Tebar F, Jaumot M, Agell N. CaM interaction and Ser181 phosphorylation as new K-Ras signaling modulators. Small GTPases 2011; Mar; 2(2): 99-103.

Bosch M, Marí M, Herms A, Fernández A, Fajardo A, Kassan A, Giralt A, Colell A, Balgoma D, Barbero E, González-Moreno E, Matias N, Tebar F, Balsinde J, Camps M, Enrich C, Gross SP, García-Ruiz C, Pérez-Navarro E, Fernández-Checa JC, Pol A. Caveolin-1 deficiency causes cholesterol-dependent mitochondrial dysfunction and apoptotic susceptibility. Curr Biol 2011; Apr 26;21(8): 681-6. doi: 10.1016/ j.cub.2011.03.030.

Aranda JF, Reglero-Real N, Kremer L, Marcos-Ramiro B, Ruiz-Sáenz A, Calvo M, Enrich C, Correas I, Millán J, Alonso MA. MYADM regulates Rac1 targeting to ordered membranes required for cell spreading and migration. Mol Biol Cell 2011; Apr 15;22(8): 1252-62. doi: 10.1091/ mbc.E10-11-0910.

Enrich C, Rentero C, de Muga SV, Reverter M, Mulay V, Wood P, Koese M, Grewal T. Annexin A6-Linking Ca(2+) signaling with cholesterol transport. Biochim Biophys Acta 2011; May;1813(5): 935-47. doi: 10.1016/ j.bbamcr. 2010.09.015.

Le Lay S, Rodriguez M, Jessup W, Rentero C, Li Q, Cartland S, Grewal T, Gaus K. Caveolin-1-mediated apolipoprotein A-I membrane binding sites are not required for cholesterol efflux. PLoS One 2011; 6(8): e23353. doi: 10.1371/ journal.pone.0023353.

  • Congresses Presentations (oral and posters)

 

Role of Annexin A6 in hepatic energetic metabolism in mice
ANNEXINS 2019. 10th International Conference on Annexin Biology
Carles Rentero, Patricia Blanco-Muñoz, Elsa Meneses-Salas, Thomas Grewal and Carlos Enrich
Poster and Oral Communication. *Short talk award to Carles Rentero

Annexin A6-mediated control of endosomal cholesterol export via TBC1D15/Rab7/StARD3 axis and its multiple cellular consequences
ANNEXINS 2019. 10th International Conference on Annexin Biology
Thomas Grewal, Jaimy Jose, Monira Hoque, Carles Rentero and Carlos Enrich
Invited speaker

AnxA6-mediated trafficking of SNAT4 regulates gluconeogenesis during liver regeneration
Patricia Blanco-Muñoz, Anna Garcia-Guaita, Elsa Meneses-Salas, Thomas Grewal, Carlos Enrich and Carles Rentero
CONSOLIDER-COAT-Annual Meeting 2019
Sant Feliu de Guixols, 28-29 May 2019
Poster presentation

Transmission electron microscopy reveals dramatic hepatic zonal changes upon chronic alcohol feeding
Raquel Fucho, Estel Solsona-Vilarrasa, Carlos Enrich, Carmen García-Ruiz and José C Fernández-Checa
EASL 2019. Vienna
Poster Presentation

The AnxA6/TBC1D15 complex controls Rab7-dependent late endosomal positioning and cholesterol export in NPC1 mutant cells
Elsa Meneses-Salas, Ana Garcia-Melero, Patricia Blanco-Muñoz, Thomas Grewal, Carles Rentero and Carlos Enrich
ASCB/EMBO 2018 meeting. San Diego CA. USA.
Poster Presentation.

The scaffold protein Annexin A6 modulates the sensitivity of EGFR overexpressing cells towards EGFR tyrosine kinase inhibitors
Yasmin A. Elmaghrabi, Monira Hoque, Paul Timpson, Carles Rentero, Carlos Enrich, Thomas Grewal
9th Garvan Signalling Symposium, 12-13 November 2018, The Garvan Institute of Medical Research, Sydney, Australia.
Poster Presentation.

The scaffold protein Annexin A6 modulates the sensitivity of EGFR overexpressing cells towards EGFR tyrosine kinase inhibitors
Yasmin A. Elmaghrabi, Monira Hoque, Paul Timpson, Carles Rentero, Carlos Enrich, Thomas Grewal
The Sydney Cancer Conference 2018, Bridging research and practice, 11-12 October 2018, ICC Sydney, Australia
Poster Presentation.

Annexin A6 a novel gatekeeper to regulate the intracelular cholesterol homeostasis
Carlos Enrich, Elsa Meneses-Salas, Elina Ikonen, Thomas Grewal and Carles Rentero
The 2018 Golgi meeting: Membrane trafficking in cell organization and homeostasis
Sorrento, Italy, 15-19 October 2018. Poster Presentation

Role of Annexin A6, a calcium binding protein, in hepatic energetic metabolism in mice
Patricia Blanco-Muñoz, Anna Alvarez-Guaita, Elsa Meneses-Salas, Thomas Grewal, Carlos Enrich and Carles Rentero
XV International Meeting of the European Calcium Society
Hamburg, Germany, 9-13 September 2018.
Selected for Oral presentation

Annexin A6 depletion rescues cholesterol egress in NPC1 mutant cells via formation of endosome-ER membrane contact sites
Thomas Grewal, Elsa Meneses-Salas, Ana Garcia-Melero, Patricia Blanco-Muñoz, Antonia Egert,  Syed S. Beevi, Carles Rentero and Carlos Enrich
XV International Meeting of the European Calcium Society
Hamburg, Germany, 9-13 September 2018
Selected for Oral presentation.

Annexin A6 depletion rescues cholesterol egress in NPC1 mutant cells via endosome-ER membrane contact formation
Thomas Grewal, Elsa Meneses-Salas, Ana Garcia-Melero, Patricia Blanco-Muñoz, Antonia Egert,  Syed S. Beevi, Carles Rentero and Carlos Enrich
59th International Conference on the Bioscience of Lipids (ICBL)
Lipid fluxes and metabolism – from fundamental mechanism to human disease
Helsinki, Finland, 4-7 September 2018
Poster Presentation.

Annexin A6 silencing reduces LE-cholesterol accumulation in NPC1 mutant cells
Elsa Meneses-Salas, Ana García-Melero, Patricia Blanco-Muñoz, Thomas Grewal, Carles Rentero and Carlos Enrich
CONSOLIDER-COAT-Annual Meeting 2018
Madrid, 7-8 March 2018
Poster Presentation

Glucose metabolism in Annexin A6 knock-out mice
Patricia Muñoz-Blanco, Anna Alvarez-Guaita, Elsa Meneses-Salas, Thomas Grewal, Carlos Enrich and Carles Rentero
CONSOLIDER-COAT-Annual Meeting 2018
Madrid, 7-8 March 2018
Poster Presentation

 

  • Invited lectures

 

XXXIII Annual Meeting of Sociedad de Biología Celular de Chile
Symposium: Lipids homeostasis and cell signalling
Recent advances of intracellular cholesterol transport and homeostasis
Puerto Varas (Chile). 25-29 November 2019
Invited speaker

ANNEXINS 2019. 10th International Conference on Annexin Biology
Annexin A6 depletion induces formation of membrane contact sites between endolysosomes and the endoplasmic reticulum in NPC1 mutant cells
Münster (Germany), September 2019.
Invited Speaker, Chair of session and Member of Scientific Board

Pontificia Universidad Católica de Chile. Facultad de Ciencias Biológicas
Santiago de Chile. Chile
Regulación del transporte intracelular del colesterol: consecuencias en la migración y fisiología celular
Conferencia Invitada. Abril 2018.

CONSOLIDER-COAT-Annual Meeting 2018
Annexin A6 a novel gatekeeper to regulate the intracellular cholesterol homeostasis
Madrid, 7-8 March 2018
Invited speaker

 

  • Seminars given (invited)

 

CONSOLIDER-COAT-Annual Meeting 2019
Annexin A6 regulates Rab7 activity and LE-Chol transport
Sant Feliu de Guixols, 28-29 May 2019
Invited speaker

Facultat de Medicina i Ciències de la Salut. Universitat de Barcelona
XIV Premi Gemma Rosell i Romero. X Setmana de la Recerca
“Mecanismes de transport intracel·lular del colesterol: canvi de paradigma i potencial terapèutic”
Conferencia invitada. Septiembre 2018

Pontificia Universidad Católica de Chile. Facultad de Medicina
Santiago de Chile. Chile
Los misterios de la regeneración hepática
Conferencia Invitada. Abril 2018

 

  • Press releases: informative articles and reviews press

 

Summary of press comments on the article published in Cell Mol Life Sci, (2019):
Nova diana terapèutica contra les malalties causades per l’acumulació de lípids a les cèl·lules
https://www.ub.edu/web/ub/ca/
https://www.ub.edu/web/ub/ca/menu_eines/noticies/2019/11/053.html

Nueva diana terapéutica contra las enfermedades causadas por la acumulación de lípidos en las células
https://www.ub.edu/web/ub/es/index.html
https://www.ub.edu/web/ub/es/menu_eines/noticies/2019/11/053.html

A new therapeutic target against diseases caused by lipid accumulation in cells
https://www.ub.edu/web/ub/en/index.html
https://www.ub.edu/web/ub/en/menu_eines/noticies/2019/11/053.html

University of Sydney
https://sydney.edu.au/medicine-health/news-and-events/2019/11/18/researchers-overcome-cholesterol-overload-in-rare-genetic-disord.html

Summary of press comments on the article published in Cell Reports, 2014:
https://www.dropbox.com/s/x4bvbkxqdunmwf0/DOSSIER%20DE%20PREMSA%20-%20CELL%20REPORTS.pdf?dl=0
https://www.dropbox.com/s/h15a68vfsj4aghk/0000_ATEI_Colesterol_CAT_revisar5.mp4?dl=0
http://www.ub.edu/ubtv/video/migracion-celular

El Pais: http://sociedad.elpais.com/sociedad/2014/05/20/actualidad/1400608401_985898.html
https://www.ub.edu/web/ub/ca/menu_eines/noticies/2014/08/04.html

Revista Ronda Iberia. July 2014

El colesterol y la migración celular
NATIONAL GEOGRAPHIC. Explora Ciencia. Junio 2015.
http://www.nationalgeographic.com.es/ciencia/el-colesterol-y-la-migracion-celular_9295

Microscòpia de superresolució. Més enllà de les lleis de la física
Carles Rentero, Anna Álvarez-Guaita, Meritxell Reverter i Carles Enrich
OMNIS CELLULA (2012) 28, 10-15.

  • Offers from the University of Barcelona:

Work UB

  • Offers from the Research Group:

At the moment, there are no job offers from this Research Group

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