Laboratory of Neural Stem Cells and Brain Damage. PI: Daniel Tornero Prieto

Presentation

The group uses human Neural Stem Cells for neurological disease modelling and the development of cell therapies for brain stroke. We are mainly interested in the functional aspects of neuronal network’s development applied to the in vitro models using brain-on-chip technology and to the functional integration of neural progenitors grafted in the damaged brain using animal models. We take advantage of top technological advances, including monosynaptic anterograde tracing with pseudotyped-rabies virus and monitorization of neuronal activity with genetically encoded calcium indicators, together with a wide network of national and international collaborators to develop novel advanced therapies that combines genetics and stem cell-based approaches.The group uses human Neural Stem Cells for neurological disease modelling and the development of cell therapies for brain stroke. We are mainly interested in the functional aspects of neuronal network’s development applied to the in vitro models using brain-on-chip technology and to the functional integration of neural progenitors grafted in the damaged brain using animal models. We take advantage of top technological advances, including monosynaptic anterograde tracing with pseudotyped-rabies virus and monitorization of neuronal activity with genetically encoded calcium indicators, together with a wide network of national and international collaborators to develop novel advanced therapies that combines genetics and stem cell-based approaches.

Daniel Tornero Prieto
Assistant Professor
daniel.tornero@ub.edu
https://twitter.com/NSC_BrainDamage
http://www.neurociencies.ub.edu/daniel-tornero/

 

Clelia Introna
PhD student
c.introna@ub.edu

 

Berta María Coll San Martín
Undergraduate researcher
bertacoll@ub.edu

 

Anna-Christina Haeb
PhD student
haeb.anna@web.de

 

Ainhoa Arcas Márquez
PhD student
ainhoaa2698@gmail.com

 

Auba Fuster
TFG student
aubafp@gmail.com

 

Claudia Gómez Bravo
TFM student
clauuuss9@gmail.com

  • Neuronal replacement in stem cell therapies for stroke-damaged brain.
  • Functional studies in neuronal networks using intracellular calcium imaging.
  • Brain-on-chip models to mimic healthy and diseased neuronal connectivity.
  • Gene editing in neural stem cells for the generation of brain disease in vitro models.

In vivo reprogramming to rescue alterations in Huntington’s disease

Project reference: HR21-00622
Principal investigator: Josep Maria Canals Coll
Affiliation entity: Universitat de Barcelona
Funding entity: Fundació “La Caixa”
Period: 01/11/2021 - 31/10/2024
Funding received: 994.890 €

Improving Functional Integration of hiPSC-derived neural progenitors transplanted into Ischemic Stroked-Brain

Project reference: PID2020-118120RB-I00
Principal investigator: Daniel Tornero Prieto
Affiliation entity: Universitat de Barcelona
Funding entity: Ministerio de Ciencia e Innovación
Period: 01/09/2021 - 31/08/2024
Funding received: 169.400 €

Neuronal networks from Cortical human iPSCs for Machine Learning Processing (NEU-ChiP)

Project reference: 964877
Principal investigator: Daniel Tornero Prieto and Jordi Soriano Fradera
Affiliation entity: Universitat de Barcelona (Fundació Bosch i Gimpera)
Funding entity: European Union FET-Open
Period: 01/09/2021 - 31/08/2024
Funding received: 3.461.780 €

For more information for PI publications click in the link:
ORCID: https://orcid.org/0000-0002-4812-4091
ResearcherID: https://publons.com/researcher/1292489/daniel-tornero/
Scopus Author ID: https://www.scopus.com/authid/detail.uri?authorId=6506127151

 

Palma-Tortosa S, Coll-San Martin B, Kokaia Z, Tornero D. Neuronal Replacement in Stem Cell Therapy for Stroke: Filling the Gap. Front Cell Dev Biol. 2021 Apr 6;9: 662636. doi: 10.3389/ fcell.2021. 662636. eCollection 2021. PMID: 33889578

Memanishvili T; Monni E; Tatarishvili J; Lindvall O; Tsiskaridze A; Kokaia Z; Tornero D. Poly(ester amide) microspheres are efficient vehicles for long-term intracerebral growth factor delivery and improve functional recovery after stroke. Biomed Mater. 2020 Nov 21; 15(6): 065020. doi: 10.1088/ 1748-605X/ aba4f6. PMID: 32650328

Grønning Hansen M; Laterza C; Palma-Tortosa S; Kvist G; Monni E; Tsupykov O; Tornero D; Uoshima N; Soriano J; Bengzon J; Martino G; Skibo G; Lindvall O; Kokaia Z. Grafted human pluripotent stem cell-derived cortical neurons integrate into adult human cortical neural circuitry. Stem Cells Transl Med. 2020. Nov;9(11): 1365-1377. doi: 10.1002/ sctm.20-0134. Epub 2020 Jun 29. PMID: 32602201

Palma-Tortosa S; Tornero D; Grønning Hansen M; Monni E; Hajy M; Kartsivadze S; Aktay S; Tsupykov O; Parmar M; Deisseroth K; Skibo G; Lindvall O; Kokaia Z. Activity in grafted human iPS cell-derived cortical neurons integrated in stroke-injured rat brain regulates motor behavior. Proc Natl Acad Sci USA. 2020 Apr 21; 117(16): 9094-9100. doi: 10.1073/ pnas.2000690117. Epub 2020 Apr 6. PMID: 32253308

Teller, S; Estevez-Priego, E; Granell, C; Tornero, D; Andilla, J; Olarte, OE; Loza-Alvarez, P; Arenas, A; Soriano, J. Spontaneous Functional Recovery after Focal Damage in Neuronal Cultures. eNeuro, 2020 Jan 3;7(1): ENEURO.0254-19.2019. doi: 10.1523/ ENEURO. 0254-19.2019. Print Jan/Feb 2020. PMID: 31818830

Ge R; Tornero D; Hirota M; Monni E; Laterza C; Lindvall O; Kokaia Z. Choroid plexus-cerebrospinal fluid route for monocyte-derived macrophages after stroke. J Neuroinflammation. 2017 Jul 28; 14(1): 153. doi: 10.1186/ s12974-017-0909-3. PMID: 28754163

de la Rosa-Prieto C; Laterza C; Gonzalez-Ramos A; Wattananit S; Ge R; Lindvall O; Tornero D; Kokaia Z. Stroke alters behavior of human skin-derived neural progenitors after transplantation adjacent to neurogenic area in rat brain. Stem Cell Res Ther. 2017 Mar 9; 8(1): 59. doi: 10.1186/ s13287-017-0513-6. PMID: 28279192

Tornero D; Tsupykov O; Granmo M; Rodriguez C; Grønning-Hansen M; Thelin J; Smozhanik E; Laterza C; Wattananit S; Ge R; Tatarishvili J; Grealish S; Brüstle O; Skibo G; Parmar M; Schouenborg J; Lindvall O; Kokaia Z. Synaptic inputs from stroke-injured brain to grafted human stem cell-derived neurons activated by sensory stimuli. Brain. 2017 Mar 1; 140(3): 692-706. doi: 10.1093/ brain/aww347. PMID: 28115364

Kokaia Z; Tornero D; Lindvall O. Transplantation of reprogrammed neurons for improved recovery after stroke. Prog Brain Res. 2017; 231:245-263. doi: 10.1016/ bs.pbr. 2016.11.013. Epub 2017 Jan 6. PMID: 28554399

Wattananit S; Tornero D; Graubardt N; Memanishvili T; Monni E; Tatarishvili J; Miskinyte G; Ge R; Ahlenius H; Lindvall O; Schwartz M; Kokaia Z. Monocyte-Derived Macrophages Contribute to Spontaneous Long-Term Functional Recovery after Stroke in Mice. J Neurosci. 2016 Apr 13; 36(15): 4182-95. doi: 10.1523/ JNEUROSCI.4317-15.2016. PMID: 27076418

Memanishvili T; Kupatadze N; Tugushi D; Katsarava R; Wattananit S; Hara N; Tornero D; Kokaia Z. Generation of cortical neurons from human induced-pluripotent stem cells by biodegradable polymeric microspheres loaded with priming factors. Biomedical Materials, 2016 Mar 23; 11(2): 025011. doi: 10.1088/ 1748-6041/ 11/2/025011. PMID: 27007569

Tornero D, Wattananit S, Grønning Madsen M, Koch P, Wood J, Tatarishvili J, Mine Y, Ge R, Monni E, Devaraju K, Hevner RF, Brüstle O, Lindvall O, Kokaia Z. Human induced pluripotent stem cell-derived cortical neurons integrate in stroke-injured cortex and improve functional recovery. Brain. 2013 Dec; 136 (Pt 12): 3561-77. doi: 10.1093/ brain/awt278. Epub 2013 Oct 21. PMID: 24148272

Oki K, Tatarishvili J, Wood J, Koch P, Wattananit S, Mine Y, Monni E, Tornero D, Ahlenius H, Ladewig J, Brüstle O, Lindvall O, Kokaia Z. Human-induced pluripotent stem cells form functional neurons and improve recovery after grafting in stroke-damaged brain. Stem Cells. 2012 Jun; 30(6): 1120-33. doi: 10.1002/ stem.1104. PMID: 22495829

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