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Molecular Biology of Reproduction and Development



Rafael Oliva Virgili


Molecular Biology of Reproduction and Development team leader

  • Professional Addresses:
    Molecular Biology of Reproduction and Development Research Group (IDIBAPS), Genetics Unit, Department of Biomedicine, Faculty of Medicine, Casanova 143, 08036 Barcelona, Spain.
    Molecular Genetics Section, Biochemistry and Molecular Genetics Service, Hospital Clínic, Villarroel 170, 08036 Barcelona, Spain.
  • Phone: +0034 93 4021877; +0034 93 2275510
  • E-mails: roliva@ub.edu / roliva@clinic.ub.es


  • Senior Consultor Geneticist (1996-Present) at the Biochemistry and Molecular Genetics Service, Hospital Clínic, Barcelona.
  • Full Professor (Catedrático; 2011-Present) at the Faculty of Medicine, University of Barcelona.
  • Member of the Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS; http://www.idibaps.org/en_index.html).
  • Previous positions:
  • Medical Doctor 1984, Faculty of Medicine, University of Barcelona.
  • PhD Student 1984-1986 at the Molecular Genetics Laboratory, Faculty of Medicine, University of Barcelona.
  • Post-Doctoral 1986-1989 at the Departament of Medical Biochemistry, University of Calgary, Canada.
  • Staff Scientist 1989-1990 at the Human Genome Center, Lawrence Berkeley Laboratories, California, USA.
  • Associate Professor 1990-2001 at the Faculty of Medicine, University of Barcelona.
  • Professor Titular 2001-2011 at the Faculty of Medicine, University of Barcelona.

Author and Research Identifiers:
ORCID (0000-0003-4876-2410): http://orcid.org/0000-0003-4876-2410
ResearcherID (C-9741-2009): http://www.researcherid.com/rid/C-9741-2009
GoogleScholarprofile: http://scholar.google.es/citations?user=uE5tV8wAAAAJ&hl=es
ScopusAuthor ID (17635957400):
    Main current Funded Projects

    Análisis proteómico y transcriptómico del semen e identificación de biomarcadores capaces de evaluar la contribución paterna a la fertilización y la embriogénesis Ministerio de economia y Competitividad -ISCIII. (Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS)). ISCIII PI16/00346, 01/01/2017-31/12/2019.
    “Reproductive Biology Early Research Training” network (REPRO-TRAIN), Marie Curie Initial Training Networks (ITN). EU- FP7-2011-ITN-289880. From 01/01/2012 until 31/12/2015.
    “Sperm cell differential proteomics and identification of diagnostic and prognostic biomarkers in assisted reproduction”. Ministerio de Economia y Competitividad-Spain-PI13/00699. From 01/01/2014 until 31/12/2016.

Sperm cell proteome and identification of diagnostic and prognostic biomarkers in infertile patients Euvitro, S.L.U. (Clínica Eugin). Rafael Oliva Virgili. (Universitat de Barcelona). 2014-01/09/2018.
Human resources obtained in the last 5 years (3 postdoctoral, 7 predoctorals). The currently active human resources in the team are the following:
Predoctoral FPU15/02306. (01/10/2016- 01/10/2020); Predoctoral PFIS-ISCIII FI17/00224. (01/01/2018
- 31/12/2021); Predoctoral Colfuturo PCB2017-9457. (2017-2020); Postdoctoral Sara Borrell - AES–ISCIII.
CD17/00109. (01/01/2018-31/12/2020);Postdoctoral Generalitat de Catalunya (PERIS) SLT002/16/00337.


140: Barrachina F, Jodar M, Delgado-Dueñas D, Soler-Ventura A, Estanyol JM, Mallofré C, Ballesca JL, Oliva R.
Stable-protein Pair Analysis as A Novel Strategy to Identify Proteomic Signatures: Application To Seminal Plasma From Infertile Patients.
Mol Cell Proteomics. 2019 Mar 15;18(Suppl 1):S77-S90. doi: 10.1074/mcp.RA118.00124

139: Requena J, Alvarez Palomo AB, Codina-Pascual M, Delgado-Morales R, Moran S, Esteller M, Sal M, Juan-Otero M, Boronat Barado A, Consiglio A, Addeleccia Bogle O, Wolvertang E, Ovchinnikov D, Alvarez I, Jaraquemada D, Mezquita-Pla J, Oliva R, Edel M.
Global proteomic and methylome analysis in human induced pluripotent stem cells reveals overexpression of a human TLR3 affecting proper innate immune response signaling.
Stem Cells, 2019 Jan 3. doi: 10.1002/stem.2966

138: Alonso-Cerezo MC, Calero Ruiz M, Chantada-Abal V, de la Fuente-Hernández LA, García-Cobaleda I, García-Ochoa C, García-Sagredo JM, Nuñez R, Oliva R, Orera-Clemente M, Pintado-Vera D, Sanchez-Ramon S.
Recommendations regarding the genetic and immunological study of reproductive dysfunction.
Med Clin (Barc). 2018 Aug 22;151(4):161.e1-161.e12. doi: 10.1016/j.medcli.2018.02.008

137: Castillo J, Jodar M, Oliva R.
The contribution of human sperm proteins to the development and epigenome of the preimplantation embryo.
Hum Reprod Update 2018; 24, 535-555

136: Soler-Ventura A, Castillo J, de la Iglesia A, Jodar M, Barrachina F, Ballesca JL, Oliva R.
A Step-By-Step Protocol For Mammalian Sperm Protamine Extraction And Analysis.
Protein Pept Lett

135: Barrachina F, Anastasiadi D, Jodar M, Castillo J, Estanyol JM, Pifarrer F, Oliva R.
Identification of a complex population of chromatin-associated proteins in addition to protamines in the European sea bass (Dicentrarchus labrax) sperm.
Systems Biology in Reproductive Medicine 2018; 64:502-517

134: Jodar M, Soler-Ventura A, Oliva R.
Semen proteomics and male infertility.
J Proteomics. 2017 Jun 6;162:125-134

133: Torabi F, Bogle OA, Estanyol JM, Oliva R, Miller D.
Zona pellucida-binding protein 2 (ZPBP2) and several proteins containing BX7B motifs in human sperm may have hyaluronic acid binding or recognition properties.
Mol Hum Reprod. 2017 Dec 1;23(12):803-816

132: Bogle OA, Kumar K, Attardo-Parrinello C, Lewis SE, Estanyol JM, Ballescà JL, Oliva R.
Identification of protein changes in human spermatozoa throughout the cryopreservation process.
Andrology. 2017 Jan;5(1):10-22

131: Carrell DT, Aston KI, Oliva R, Emery BR, De Jonge CJ.
The "omics" of human male infertility: integrating big data in a systems biology approach.
Cell Tissue Res. 2016;363:295-312.

130: Castillo J, Estanyol JM, Ballescá JL, Oliva R.
Human sperm chromatin epigenetic potential: genomics, proteomics, and male infertility.
Asian J Androl. 2015;17:601-9.

129: Codina M, Estanyol JM, Fidalgo MJ, Ballescà JL, Oliva R.
Advances in sperm proteomics: best-practise methodology and clinical potential.
Expert Rev Proteomics. 2015;12:255-77.

128: Pantano L, Jodar M, Bak M, Ballescà JL, Tommerup N, Oliva R, Vavouri T.
The small RNA content of human sperm reveals pseudogene-derived piRNAs complementary to protein-coding genes.
RNA. 2015;21:1085-95.

127: Paiva C, Amaral A, Rodriguez M, Canyellas N, Correig X, Ballescà JL, Ramalho-Santos J, Oliva R.
Identification of endogenous metabolites in human sperm cells using proton nuclear magnetic resonance ((1) H-NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS).
Andrology. 2015;3:496-505.

126: Vilagran I, Yeste M, Sancho S, Castillo J, Oliva R, Bonet S.
Comparative analysis of boar seminal plasma proteome from different freezability ejaculates and identification of Fibronectin 1 as sperm freezability marker.
Andrology. 2015;3:345-56.

125: Amaral A, Paiva C, Attardo Parrinello C, Estanyol JM, Ballescà JL, Ramalho-Santos J, Oliva R.
Identification of proteins involved in human sperm motility using high-throughput differential proteomics.
J Proteome Res. 2014;13:5670-84.

124: Castillo J, Amaral A, Azpiazu R, Vavouri T, Estanyol JM, Ballescà JL, Oliva R.
Genomic and proteomic dissection and characterization of the human sperm chromatin.
Mol Hum Reprod. 2014;20:1041-53.

123: Zatecka E, Castillo J, Elzeinova F, Kubatova A, Ded L, Peknicova J, Oliva R.
The effect of tetrabromobisphenol A on protamine content and DNA integrity in mouse spermatozoa.
Andrology. 2014;2:910-7.

122: Azpiazu R, Amaral A, Castillo J, Estanyol JM, Guimerà M, Ballescà JL, Balasch J, Oliva R.
High-throughput sperm differential proteomics suggests that epigenetic alterations contribute to failed assisted reproduction.
Hum Reprod. 2014;29:1225-37.

121: Castillo J, Amaral A, Oliva R.
Sperm nuclear proteome and its epigenetic potential.
Andrology. 2014;2:326-38.

120: Jodar M, Oliva R.
Protamine alterations in human spermatozoa.
Adv Exp Med Biol. 2014;791:83-102.

119: Amaral A, Castillo J, Ramalho-Santos J, Oliva R.
The combined human sperm proteome: cellular pathways and implications for basic and clinical science.
Hum Reprod Update. 2014;20:40-62.

118: Giacco DL, Chianese C, Sánchez-Curbelo J, Bassas L, Ruiz P, Rajmil O, Sarquella J, Vives A, Ruiz-Castañé E, Oliva R, Ars E, Krausz C.
Clinical relevance of Y-linked CNV screening in male infertility: new insights based on the 8-year experience of a diagnostic genetic laboratory.
Eur J Hum Genet. 2014;22:754-61

117: Vilagran I, Castillo J, Bonet S, Sancho S, Yeste M, Estanyol JM, Oliva R.
Acrosin-binding protein (ACRBP) and triosephosphate isomerase (TPI) are good markers to predict boar sperm freezing capacity.
Theriogenology. 2013;80:443-50.

116: de Mateo S, Estanyol JM, Oliva R.
Methods for the analysis of the sperm proteome.
Methods Mol Biol. 2013;927:411-22

115: Amaral A, Castillo J, Estanyol JM, Ballesca JL, Ramalho-Santos J, Oliva R.
Human sperm tail proteome suggests new endogenous metabolic pathways.
Mol Cell Proteomics. 2013 Feb;12(2):330-42.

114: Oliva R.
SBiRM: Focus on proteomics and reproduction. Preface.
Syst Biol Reprod Med. 2012;58:177-8.

113: Jodar M, Kalko S, Castillo J, Ballescà JL, Oliva R.
Differential RNAs in the sperm cells of asthenozoospermic patients.
Hum Reprod. 2012;27:1431-8.

112: Oliva R, Ballescà JL.
Altered histone retention and epigenetic modifications in the sperm of infertile men.
Asian J Androl. 2012;14:239-40.

111: Simon L, Castillo J, Oliva R, Lewis SE.
Relationships between human sperm protamines, DNA damage and assisted reproduction outcomes.
Reprod Biomed Online. 2011;23:724-34.

110: de Mateo S, Castillo J, Estanyol JM, Ballescà JL, Oliva R.
Proteomic characterization of the human sperm nucleus.
Proteomics. 2011;11:2714-26.

109: de Mateo S, Ramos L, van der Vlag J, de Boer P, Oliva R.
Improvement in chromatin maturity of human spermatozoa selected through density gradient centrifugation.
Int J Androl. 2011;34:256-67.

108: Antonell A, Balasa M, Oliva R, Lladó A, Bosch B, Fabregat N, Fortea J, Molinuevo JL, Sánchez-Valle R.
A novel PSEN1 gene mutation (L235R) associated with familial early-onset Alzheimer's disease.
Neurosci Lett. 2011 May 27;496(1):40-2.

107: Castillo J, Simon L, de Mateo S, Lewis S, Oliva R.
Protamine/DNA Ratios and DNA Damage in Native and Density Gradient Centrifuged Sperm From Infertile Patients.
J Androl. 2011;32:324-32.

106: Fortea J, Lladó A, Clarimón J, Lleó A, Oliva R, Peri J, Pintor L, Yagüe J, Blesa R, Molinuevo JL, Sánchez-Valle R.
PICOGEN: Five years experience with a genetic counselling program for dementia.
Neurologia. 2011 Apr;26(3):143-149.

105: de Mateo S, Ramos L, de Boer P, Meistrich M, Oliva R.
Protamine 2 Precursors and Processing.
Protein Pept Lett. 2011 Mar 28. [Epub ahead of print]Protamine 2 Precursors and Processing.de Mateo S, Ramos L, de Boer P, Meistrich M, Oliva R

104: Oliva R, Castillo J.
Proteomics and the genetics of sperm chromatin condensation.
Asian J Androl. 2011;13:24-30.

103: Oliva R, De Mateo S, Castillo J, Azpiazu R, Oriola J, Ballescà JL.
Methodological advances in sperm proteomics.
Hum Fertil (Camb). 2010 Dec;13(4):263-7

102: Jodar M, Oriola J, Mestre G, Castillo J, Giwercman A, Vidal-Taboada JM, Ballescà JL, Oliva R.
Polymorphisms, haplotypes and mutations in the protamine 1 and 2 genes.
Int J Androl. 2011;34:470-85

101: de Mateo S, Gázquez C, Guimerà M, Balasch J, Meistrich ML, Ballescà JL, Oliva R.
Protamine 2 precursors (Pre-P2), protamine 1 to protamine 2 ratio (P1/P2), and assisted reproduction outcome.
Fertil Steril. 2009;91:715-22.

100: Bufill E, Bartés A, Moral A, Casadevall T, Codinachs M, Zapater E, Carles Rovira J, Roura P, Oliva R, Blesa R.
[Genetic and environmental factors that may influence in the senile form of Alzheimer's disease: nested case control studies].
Neurologia. 2009 Mar;24(2):108-12

99: Oliva R, de Mateo S, Estanyol JM.
Sperm cell proteomics.
Proteomics. 2009 Feb;9(4):1004-17

98: Krausz C, Giachini C, Xue Y, O'Bryan MK, Gromoll J, Rajpert-de Meyts E, Oliva R, Aknin-Seifer I, Erdei E, Jorgensen N, Simoni M, Ballescà JL, Levy R, Balercia G, Piomboni P, Nieschlag E, Forti G, McLachlan R, Tyler-Smith C.
Phenotypic variation within European carriers of the Y-chromosomal gr/gr deletion is independent of Y-chromosomal background.
J Med Genet. 2009 Jan;46(1):21-31.

97: Gázquez C, Oriola J, de Mateo S, Vidal-Taboada JM, Ballescà JL, Oliva R.
A common protamine 1 promoter polymorphism (-190 C->A) correlates with abnormal sperm morphology and increased protamine P1/P2 ratio in infertile patients.
J Androl. 2008 Sep-Oct;29(5):540-8.

96: Queralt R, Madrigal I, Vallecillos MA, Morales C, Ballescá JL, Oliva R, Soler A, Sánchez A, Margarit E.
Atypical XX male with the SRY gene located at the long arm of chromosome 1 and a 1qter microdeletion.
Am J Med Genet A. 2008 May 15;146A(10):1335-40

95: Martínez-Heredia J, de Mateo S, Vidal-Taboada JM, Ballescà JL, Oliva R.
Identification of proteomic differences in asthenozoospermic sperm samples.
Hum Reprod. 2008 Apr;23(4):783-91.

94: Mathivanan S, Ahmed M, Ahn NG, Alexandre H, Amanchy R, Andrews PC, Bader JS, Balgley BM, Bantscheff M, Bennett KL, Björling E, Blagoev B, Bose R, Brahmachari SK, Burlingame AS, Bustelo XR, Cagney G, Cantin GT, Cardasis HL, Celis JE, Chaerkady R, Chu F, Cole PA, Costello CE, Cotter RJ, Crockett D, DeLany JP, De Marzo AM, DeSouza LV, Deutsch EW, Dransfield E, Drewes G, Droit A, Dunn MJ, Elenitoba-Johnson K, Ewing RM, Van Eyk J, Faca V, Falkner J, Fang X, Fenselau C, Figeys D, Gagné P, Gelfi C, Gevaert K, Gimble JM, Gnad F, Goel R, Gromov P, Hanash SM, Hancock WS, Harsha HC, Hart G, Hays F, He F, Hebbar P, Helsens K, Hermeking H, Hide W, Hjernø K, Hochstrasser DF, Hofmann O, Horn DM, Hruban RH, Ibarrola N, James P, Jensen ON, Jensen PH, Jung P, Kandasamy K, Kheterpal I, Kikuno RF, Korf U, Körner R, Kuster B, Kwon MS, Lee HJ, Lee YJ, Lefevre M, Lehvaslaiho M, Lescuyer P, Levander F, Lim MS, Löbke C, Loo JA, Mann M, Martens L, Martinez-Heredia J, McComb M, McRedmond J, Mehrle A, Menon R, Miller CA, Mischak H, Mohan SS, Mohmood R, Molina H, Moran MF, Morgan JD, Moritz R, Morzel M, Muddiman DC, Nalli A, Navarro JD, Neubert TA, Ohara O, Oliva R, Omenn GS, Oyama M, Paik YK, Pennington K, Pepperkok R, Periaswamy B, Petricoin EF, Poirier GG, Prasad TS, Purvine SO, Rahiman BA, Ramachandran P, Ramachandra YL, Rice RH, Rick J, Ronnholm RH, Salonen J, Sanchez JC, Sayd T, Seshi B, Shankari K, Sheng SJ, Shetty V, Shivakumar K, Simpson RJ, Sirdeshmukh R, Siu KW, Smith JC, Smith RD, States DJ, Sugano S, Sullivan M, Superti-Furga G, Takatalo M, Thongboonkerd V, Trinidad JC, Uhlen M, Vandekerckhove J, Vasilescu J, Veenstra TD, Vidal-Taboada JM, Vihinen M, Wait R, Wang X, Wiemann S, Wu B, Xu T, Yates JR, Zhong J, Zhou M, Zhu Y, Zurbig P, Pandey A.
Human Proteinpedia enables sharing of human protein data.
Nat Biotechnol. 2008 Feb;26(2):164-7

93: Oliva R, Martínez-Heredia J, Estanyol JM.
Proteomics in the study of the sperm cell composition, differentiation and function.
Syst Biol Reprod Med. 2008 Jan-Feb;54(1):23-36

92: Domínguez-Fandos D, Camejo MI, Ballescà JL, Oliva R.
Human sperm DNA fragmentation: correlation of TUNEL results as assessed by flow cytometry and optical microscopy.
Cytometry A. 2007 Dec;71(12):1011-8

91: de Mateo S, Martínez-Heredia J, Estanyol JM, Domínguez-Fandos D, Vidal-Taboada JM, Ballescà JL, Oliva R.
Marked correlations in protein expression identified by proteomic analysis of human spermatozoa.
Proteomics. 2007 Dec;7(23):4264-77

90: Oliva R, Martínez-Heredia J, de Mateo S, Gázquez C, Oriola J, Estanyol JM, Guimerà M, Balasch J, Ballescà JL.
Proteomics of human spermatozoa, protamine content and assisted reproduction outcome.
Soc Reprod Fertil Suppl. 2007;65:527-30

89: Martínez-Heredia J, Estanyol JM, Ballescà JL, Oliva R.
Proteomic identification of human sperm proteins.
Proteomics. 2006 Aug;6(15):4356-69

88: Torregrosa N, Domínguez-Fandos D, Camejo MI, Shirley CR, Meistrich ML, Ballescà JL, Oliva R.
Protamine 2 precursors, protamine 1/protamine 2 ratio, DNA integrity and other sperm parameters in infertile patients.
Hum Reprod. 2006 Aug;21(8):2084-9.

87: Oliva R.
Protamines and male infertility.
Hum Reprod Update. 2006 Jul-Aug;12(4):417-35. Epub 2006 Mar 31

86: Ribal MJ, Mengual L, Marín M, Algaba F, Ars E, Fernández PL, Oliva R, Villavicencio H, Alcaraz A.
Molecular staging of bladder cancer with RT-PCR assay for CK20 in peripheral blood, bone marrow and lymph nodes: comparison with standard histological staging.
Anticancer Res. 2006 Jan-Feb;26(1A):411-9

85: Molinuevo JL, Pintor L, Peri JM, Lleó A, Oliva R, Marcos T, Blesa R.
Emotional reactions to predictive testing in Alzheimer's disease and other inherited dementias.
Am J Alzheimers Dis Other Demen. 2005 Jul-Aug;20(4):233-8

84: de Llanos M, Ballescà JL, Gázquez C, Margarit E, Oliva R.
High frequency of gr/gr chromosome Y deletions in consecutive oligospermic ICSI candidates.
Hum Reprod. 2005 Jan;20(1):216-20.

83: Oliva R, Novials A, Sánchez M, Villa M, Ingelmo M, Recasens M, Ascaso C, Bruguera M, Gomis R.
The HFE gene is associated to an earlier age of onset and to the presence of diabetic nephropathy in diabetes mellitus type 2.
Endocrine. 2004 Jul;24(2):111-4

82: Ezquerra M, Lleó A, Castellví M, Queralt R, Santacruz P, Pastor P, Molinuevo JL, Blesa R, Oliva R.
A novel mutation in the PSEN2 gene (T430M) associated with variable expression in a family with early-onset Alzheimer disease.
Arch Neurol. 2003 Aug;60(8):1149-51

81: Sánchez M, Villa M, Ingelmo M, Sanz C, Bruguera M, Ascaso C, Oliva R.
Population screening for hemochromatosis: a study in 5370 Spanish blood donors.
J Hepatol. 2003 Jun;38(6):745-50

80: Mengual L, Ballescá JL, Ascaso C, Oliva R.
Marked differences in protamine content and P1/P2 ratios in sperm cells from percoll fractions between patients and controls.
J Androl. 2003 May-Jun;24(3):438-47

79: Repiso A, Pérez de la Ossa P, Avilés X, Oliva B, Juncá J, Oliva R, Garcia E, Vives-Corrons JL, Carreras J, Climent F.
Red blood cell phosphosphoglycerate mutase. Description of the first human BB isoenzyme mutation.
Haematologica. 2003 Mar;88(3):ECR07

78: Mengual L, Oriola J, Ascaso C, Ballescà JL, Oliva R.
An increased CAG repeat length in the androgen receptor gene in azoospermic ICSI candidates.
J Androl. 2003 Mar-Apr;24(2):279-84

77: Lleó A, Blesa R, Queralt R, Ezquerra M, Molinuevo JL, Peña-Casanova J, Rojo A, Oliva R.
Frequency of mutations in the presenilin and amyloid precursor protein genes in early-onset Alzheimer disease in Spain.
Arch Neurol. 2002 Nov;59(11):1759-63

76: Muñoz E, Tolosa E, Pastor P, Martí MJ, Valldeoriola F, Campdelacreu J, Oliva R.
Relative high frequency of the c.255delA parkin gene mutation in Spanish patients with autosomal recessive parkinsonism.
J Neurol Neurosurg Psychiatry. 2002 Nov;73(5):582-4

75: Ramos VC, Vidal-Taboada J, Bergoñon S, Egeo A, Fisher EM, Scartezzini P, Oliva R.
Characterisation and expression analysis of the WDR9 gene, located in the Down critical region-2 of the human chromosome 21.
Biochim Biophys Acta. 2002 Sep 27;1577(3):377-83

74: Lleó A, Blesa R, Gendre J, Castellví M, Molinuevo JL, Oliva R.
[Clinical characteristics of a family with early-onset Alzheimer's disease associated with a presenilin 1 mutation (M139T)].
Med Clin (Barc). 2002 May 18;118(18):698-700

73: Lleó A, Blesa R, Gendre J, Castellví M, Pastor P, Queralt R, Oliva R.
A novel presenilin 2 gene mutation (D439A) in a patient with early-onset Alzheimer's disease.
Neurology. 2001 Nov 27;57(10):1926-8

72: Pastor P, Muñoz E, Ezquerra M, Obach V, Martí MJ, Valldeoriola F, Tolosa E, Oliva R.
Analysis of the coding and the 5' flanking regions of the alpha-synuclein gene in patients with Parkinson's disease.
Mov Disord. 2001 Nov;16(6):1115-9

71: Oliva R.
[Human genome: genomics, genetics and medical applications].
Med Clin (Barc). 2001 May 12;116(17):672-5

70: Muñoz E, Pastor P, José Martí M, Valldeoriola F, Oliva R, Tolosa E.
[Sporadic and familial Parkinson's disease: comparative study].
Med Clin (Barc). 2001 May 5;116(16):601-4

69: Queralt R, Ezquerra M, Castellví M, Lleó A, Blesa R, Oliva R.
Detection of the presenilin 1 gene mutation (M139T) in early-onset familial Alzheimer disease in Spain.
Neurosci Lett. 2001 Feb 23;299(3):239-41

68: Pastor P, Pastor E, Carnero C, Vela R, García T, Amer G, Tolosa E, Oliva R.
Familial atypical progressive supranuclear palsy associated with homozigosity for the delN296 mutation in the tau gene.
Ann Neurol. 2001 Feb;49(2):263-7

67: Sánchez M, Bruguera M, Rodés J, Oliva R.
Complete characterization of the 3' region of the human and mouse hereditary hemochromatosis HFE gene and detection of novel splicing forms.
Blood Cells Mol Dis. 2001 Jan-Feb;27(1):35-43

66: Oliva R, Sánchez M, Bruguera M, Rodés J.
[Clinical usefulness of the detection of mutations of the HFE gene in hemochromatosis].
Gastroenterol Hepatol. 2000 Nov;23(9):433-5

65: Paracchini S, Stuppia L, Gatta V, Palka G, Moro E, Foresta C, Mengua L, Oliva R, Ballescà JL, Kremer JA, van Golde RJ, Tuerlings JH, Hargreave T, Ross A, Cooke H, Huellen K, Vogt PH, Tyler-Smith C.
Y-chromosomal DNA haplotypes in infertile European males carrying Y-microdeletions.
J Endocrinol Invest. 2000 Nov;23(10):671-6

64: Pérez JL, García L, Talavera A, Oliva R, Valmaseda T, Año G, Pérez O, Sierra G.
Passive protection of serum from volunteers inoculated with attenuated strain 638 of Vibrio cholerae O1 in animal models.
Vaccine. 2000 Sep 15;19(2-3):376-84

63: Vila N, Obach V, Revilla M, Oliva R, Chamorro A.
Alpha(1)-antichymotrypsin gene polymorphism in patients with stroke.
Stroke. 2000 Sep;31(9):2103-5

62: Muñoz E, Pastor P, Martí MJ, Oliva R, Tolosa E.
A new mutation in the parkin gene in a patient with atypical autosomal recessive juvenile parkinsonism.
Neurosci Lett. 2000 Jul 28;289(1):66-8

61: Obach V, Arroyo S, Santamaria J, Grinberg D, Oliva R.
No evidence of linkage to 6p markers in spanish families with juvenile myoclonic epilepsy.
Neurosci Lett. 2000 Jun 9;286(3):213-7

60: Vidal-Taboada JM, Lu A, Pique M, Pons G, Gil J, Oliva R.
Down syndrome critical region gene 2: expression during mouse development and in human cell lines indicates a function related to cell proliferation.
Biochem Biophys Res Commun. 2000 May 27;272(1):156-63

59: Ezquerra M, Carnero C, Blesa R, Oliva R.
A novel presenilin 1 mutation (Leu166Arg) associated with early-onset Alzheimer disease.
Arch Neurol. 2000 Apr;57(4):485-8

58: Molinuevo JL, Valldeoriola F, Alegret M, Oliva R, Tolosa E.
Progressive supranuclear palsy: earlier age of onset in patients with the tau protein A0/A0 genotype.
J Neurol. 2000 Mar;247(3):206-8

57: Arrufat FJ, Diaz R, Queralt R, Navarro V, Marcos T, Massana G, Massana J, Ballesta F, Oliva R.
Analysis of the polymorphic (GT)(n) repeat at the dopamine beta-hydroxylase gene in Spanish patients affected by schizophrenia.
Am J Med Genet. 2000 Feb 7;96(1):88-92

56: Pastor P, Ezquerra M, Muñoz E, Martí MJ, Blesa R, Tolosa E, Oliva R.
Significant association between the tau gene A0/A0 genotype and Parkinson's disease.
Ann Neurol. 2000 Feb;47(2):242-5

55: Margarit E, Coll MD, Oliva R, Gómez D, Soler A, Ballesta F.
SRY gene transferred to the long arm of the X chromosome in a Y-positive XX true hermaphrodite.
Am J Med Genet. 2000 Jan 3;90(1):25-8

54: Oliva R.
[Genetics of frontotemporal dementia and alterations of the tau gene].
Neurologia. 2000 Jan;15 Suppl 1:33-7

53: Sánchez M, Bruguera M, Quintero E, Barrio Y, Mazzara R, Rodés J, Oliva R.
Hereditary hemochromatosis in Spain.
Genet Test. 2000;4(2):171-6

52: Ezquerra M, Pastor P, Valldeoriola F, Molinuevo JL, Blesa R, Tolosa E, Oliva R.
Identification of a novel polymorphism in the promoter region of the tau gene highly associated to progressive supranuclear palsy in humans.
Neurosci Lett. 1999 Nov 19;275(3):183-6

51: Pastor P, Muñoz E, Obach V, Martí MJ, Blesa R, Oliva R, Tolosa E.
Dopamine receptor D2 intronic polymorphism in patients with Parkinson's disease.
Neurosci Lett. 1999 Oct 8;273(3):151-4

50: Gudayol M, Vidal-Taboada JM, Usac EF, Costa A, Cristòbal P, dell'Anna C, Oliva R, Gomis R.
Detection of a new variant of the mitochondrial glycerol-3-phosphate dehydrogenase gene in Spanish type 2 DM patients.
Biochem Biophys Res Commun. 1999 Sep 24;263(2):439-45

49: Ezquerra M, Carnero C, Blesa R, Gelpí JL, Ballesta F, Oliva R.
A presenilin 1 mutation (Ser169Pro) associated with early-onset AD and myoclonic seizures.
Neurology. 1999 Feb;52(3):566-70

48: Muñoz E, Obach V, Oliva R, Martí MJ, Ezquerra M, Pastor P, Ballesta F, Tolosa E.
Alpha1-antichymotrypsin gene polymorphism and susceptibility to Parkinson's disease.
Neurology. 1999 Jan 15;52(2):297-301

47: Ballesta F, Queralt R, Gómez D, Solsona E, Guitart M, Ezquerra M, Moreno J, Oliva R.
Parental origin and meiotic stage of non-disjunction in 139 cases of trisomy 21.
Ann Genet. 1999;42(1):11-5

46: Sánchez M, Queralt R, Bruguera M, Rodés J, Oliva R.
Cloning, sequencing and characterization of the rat hereditary hemochromatosis promoter: comparison of the human, mouse and rat HFE promoter regions.
Gene. 1998 Dec 28;225(1-2):77-87

45: Sánchez M, Bruguera M, Bosch J, Rodés J, Ballesta F, Oliva R.
Prevalence of the Cys282Tyr and His63Asp HFE gene mutations in Spanish patients with hereditary hemochromatosis and in controls.
J Hepatol. 1998 Nov;29(5):725-8

44: Vidal-Taboada JM, Sanz S, Egeo A, Scartezzini P, Oliva R.
Identification and characterization of a new gene from human chromosome 21 between markers D21S343 and D21S268 encoding a leucine-rich protein.
Biochem Biophys Res Commun. 1998 Sep 29;250(3):547-54

43: Oliva R, Margarit E, Ballescá JL, Carrió A, Sánchez A, Milà M, Jiménez L, Alvarez-Vijande JR, Ballesta F.
Prevalence of Y chromosome microdeletions in oligospermic and azoospermic candidates for intracytoplasmic sperm injection.
Fertil Steril. 1998 Sep;70(3):506-10

42: Margarit E, Soler A, Carrió A, Oliva R, Costa D, Vendrell T, Rosell J, Ballesta F.
Molecular, cytogenetic, and clinical characterisation of six XX males including one prenatal diagnosis.
J Med Genet. 1998 Sep;35(9):727-30

41: Oliva R, Tolosa E, Ezquerra M, Molinuevo JL, Valldeoriola F, Burguera J, Calopa M, Villa M, Ballesta F.
Significant changes in the tau A0 and A3 alleles in progressive supranuclear palsy and improved genotyping by silver detection.
Arch Neurol. 1998 Aug;55(8):1122-4

40: Bench G, Corzett MH, De Yebra L, Oliva R, Balhorn R.
Protein and DNA contents in sperm from an infertile human male possessing protamine defects that vary over time.
Mol Reprod Dev. 1998 Jul;50(3):345-53

39: Egeo A, Mazzocco M, Arrigo P, Vidal-Taboada JM, Oliva R, Pirola B, Giglio S, Rasore-Quartino A, Scartezzini P.
Identification and characterization of a new human gene encoding a small protein with high homology to the proline-rich region of the SH3BGR gene.
Biochem Biophys Res Commun. 1998 Jun 18;247(2):302-6

38: Ezquerra M, Ballesta F, Queralt R, Aledo R, Gómez D, Guitart M, Egozcue J, Ascaso C, Oliva R.
Apolipoprotein E epsilon 4 alleles and meiotic origin of non-disjunction in Down syndrome children and in their corresponding fathers and mothers.
Neurosci Lett. 1998 May 22;248(1):1-4

37: Margarit E, Guillén A, Rebordosa C, Vidal-Taboada J, Sánchez M, Ballesta F, Oliva R.
Identification of conserved potentially regulatory sequences of the SRY gene from 10 different species of mammals.
Biochem Biophys Res Commun. 1998 Apr 17;245(2):370-7

36: de Yebra L, Ballescá JL, Vanrell JA, Corzett M, Balhorn R, Oliva R.
Detection of P2 precursors in the sperm cells of infertile patients who have reduced protamine P2 levels.
Fertil Steril. 1998 Apr;69(4):755-9

35: Egeo A, Mazzocco M, Sotgia F, Arrigo P, Oliva R, Bergonòn S, Nizetic D, Rasore-Quartino A, Scartezzini P.
Identification and characterization of a new human cDNA from chromosome 21q22.3 encoding a basic nuclear protein.
Hum Genet. 1998 Mar;102(3):289-93

34: Vidal-Taboada JM, Bergoñón S, Sánchez M, López-Acedo C, Groet J, Nizetic D, Egeo A, Scartezzini P, Katsanis N, Fisher EM, Delabar JM, Oliva R.
High resolution physical mapping and identification of transcribed sequences in the Down syndrome region-2.
Biochem Biophys Res Commun. 1998 Feb 13;243(2):572-8

33: Aldudo J, Bullido MJ, Arbizu T, Oliva R, Valdivieso F.
Identification of a novel mutation (Leu282Arg) of the human presenilin 1 gene in Alzheimer's disease.
Neurosci Lett. 1998 Jan 16;240(3):174-6

32: Ezquerra M, Blesa R, Tolosa E, Ballesta F, Oliva R.
Alpha-antichymotrypsin gene polymorphism and risk for Alzheimer's disease in the Spanish population.
Neurosci Lett. 1998 Jan 9;240(2):107-9

31: Lopez OL, Lopez-Pousa S, Kamboh MI, Adroer R, Oliva R, Lozano-Gallego M, Becker JT, DeKosky ST.
Apolipoprotein E polymorphism in Alzheimer's disease: a comparative study of two research populations from Spain and the United States.
Eur Neurol. 1998;39(4):229-33

30: Vidal-Taboada JM, Bergoñon S, Scartezzini P, Egeo A, Nizetic D, Oliva R.
High-resolution physical map and identification of potentially regulatory sequences of the human SH3BGR located in the Down syndrome chromosomal region.
Biochem Biophys Res Commun. 1997 Dec 18;241(2):321-6

29: Muñoz E, Oliva R, Obach V, Martí MJ, Pastor P, Ballesta F, Tolosa E.
Identification of Spanish familial Parkinson's disease and screening for the Ala53Thr mutation of the alpha-synuclein gene in early onset patients.
Neurosci Lett. 1997 Oct 10;235(1-2):57-60

28: Gené M, Moreno P, Ezquerra M, Prat A, Huguet E, Adroer R, Oliva R.
Low apolipoprotein E epsilon4 allele frequency in the population of Catalonia (Spain) determined by PCR-RFLP and Laser fluorescent sequencer.
Eur J Epidemiol. 1997 Oct;13(7):841-3

27: Ezquerra M, Blesa R, Tolosa E, Lopez Pousa S, Aguilar M, Peña J, Van Broeckhoven C, Ballesta F, Oliva R.
The genotype 2/2 of the presenilin-1 polymorphism is decreased in Spanish early-onset Alzheimer's disease.
Neurosci Lett. 1997 May 23;227(3):201-4

26: Adroer R, López-Acedo C, Oliva R.
Conserved elements in the 5' regulatory region of the amyloid precursor protein gene in primates.
Neurosci Lett. 1997 May 2;226(3):203-6

25: Estupiñán B, Alvarez R, Cuesta T, Fernández I, Almeida A, Subiaul Z, Melendy C, Oliva R, Alvarez L.
[Clinico-pathological correlation in the main types of dementia].
Rev Neurol. 1997 Apr;25(140):547-9

24: Blesa R, Adroer R, Santacruz P, Ascaso C, Tolosa E, Oliva R.
High apolipoprotein E epsilon 4 allele frequency in age-related memory decline.
Ann Neurol. 1996 Apr;39(4):548-51

23: Adroer R, Santacruz P, Blesa R, López-Pousa S, Ascaso C, Oliva R.
Apolipoprotein E4 allele frequency in Spanish Alzheimer and control cases.
Neurosci Lett. 1995 Apr 21;189(3):182-6

22: Queralt R, Oliva R.
Demonstration of trans-acting factors binding to the promoter region of the testis-specific rat protamine P1 gene.
Biochem Biophys Res Commun. 1995 Mar 17;208(2):802-12

21: Queralt R, Oliva R.
Identification of conserved potential regulatory sequences of the protamine-encoding P1 genes from ten different mammals.
Gene. 1993 Nov 15;133(2):197-204

20: Queralt R, de Fàbregues-Boixar O, Adroer R, Gené M, Gómez-Catalán J, Huguet E, Oliva R.
Direct sequencing of the human protamine P1 gene and application in forensic medicine.
J Forensic Sci. 1993 Nov;38(6):1491-501

19: Retief JD, Winkfein RJ, Dixon GH, Adroer R, Queralt R, Ballabriga J, Oliva R.
Evolution of protamine P1 genes in primates.
J Mol Evol. 1993 Oct;37(4):426-34

18: de Yebra L, Ballescà JL, Vanrell JA, Bassas L, Oliva R.
Complete selective absence of protamine P2 in humans.
J Biol Chem. 1993 May 15;268(14):10553-7

17: de Yebra L, Oliva R.
Rapid analysis of mammalian sperm nuclear proteins.
Anal Biochem. 1993 Feb 15;209(1):201-3

16: Adroer R, Lopez-Acedo C, Oliva R, Hardy J, Fidani L.
A novel silent variant at codon 711 and a variant at codon 708 of the APP sequence detected in Spanish Alzheimer and control cases.
Neurosci Lett. 1993 Feb 5;150(1):33-4

15: Adroer R, Chartier-Harlin MC, Crawford F, Oliva R.
Improved direct sequencing of Alzheimer's amyloid precursor protein (APP) exons 16 and 17.
Neurosci Lett. 1992 Jul 6;141(1):69-71

14: Adroer R, Queralt R, Ballabriga J, Oliva R.
Nucleotide sequence of the protamine P1 gene from the whale Orcinus orca predicts a unique N-terminal amino-acid motif.
Nucleic Acids Res. 1992 Feb 11;20(3):609

13: Ichikawa H, Shimizu K, Saito A, Wang DN, Oliva R, Kobayashi H, Kaneko Y, Miyoshi H, Smith CL, Cantor CR, et al.
Long-distance restriction mapping of the proximal long arm of human chromosome 21 with Not I linking clones.
Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):23-7

12: Queralt R, Oliva R.
Protamine 1 gene sequence from the primate Saguinus imperator isolated with PCR using consensus oligonucleotides.
Nucleic Acids Res. 1991 Oct 25;19(20):5786

11: Oliva R, Dixon GH.
Expression and processing of the rooster protamine mRNA.
Ann N Y Acad Sci. 1991;637:289-99

10: Oliva R, Dixon GH.
Vertebrate protamine genes and the histone-to-protamine replacement reaction.
Prog Nucleic Acid Res Mol Biol. 1991;40:25-94

9: Oliva R, Bazett-Jones DP, Locklear L, Dixon GH.
Histone hyperacetylation can induce unfolding of the nucleosome core particle.
Nucleic Acids Res. 1990 May 11;18(9):2739-47

8: Oliva R, Goren R, Dixon GH.
Quail (Coturnix japonica) protamine, full-length cDNA sequence, and the function and evolution of vertebrate protamines.
J Biol Chem. 1989 Oct 25;264(30):17627-30

7: Oliva R, Dixon GH.
Chicken protamine genes are intronless. The complete genomic sequence and organization of the two loci.
J Biol Chem. 1989 Jul 25;264(21):12472-81

6: Oliva R, Mezquita J, Mezquita C, Dixon GH.
Haploid expression of the rooster protamine mRNA in the postmeiotic stages of spermatogenesis.
Dev Biol. 1988 Feb;125(2):332-40

5: Oliva R, Bazett-Jones D, Mezquita C, Dixon GH.
Factors affecting nucleosome disassembly by protamines in vitro. Histone hyperacetylation and chromatin structure, time dependence, and the size of the sperm nuclear proteins.
J Biol Chem. 1987 Dec 15;262(35):17016-25

4: Mezquita J, Oliva R, Mezquita C.
New ubiquitin mRNA expressed during chicken spermiogenesis.
Nucleic Acids Res. 1987 Nov 25;15(22):9604

3: Oliva R, Mezquita C.
Marked differences in the ability of distinct protamines to disassemble nucleosomal core particles in vitro.
Biochemistry. 1986 Oct 21;25(21):6508-11

2: Oliva R, Mezquita C.
Histone H4 hyperacetylation and rapid turnover of its acetyl groups in transcriptionally inactive rooster testis spermatids.
Nucleic Acids Res. 1982 Dec 20;10(24):8049-59

1: Oliva R, Vidal S, Mezquita C.
Cellular content and biosynthesis of polyamines during rooster spermatogenesis.
Biochem J. 1982 Nov 15;208(2):269-73