WT1 in Cell Plasticity

Department of Cell Biology
Faculty of Biology

Physiology and Immunology

Dr. Ofelia M. Martínez Estrada
Av. Diagonal 643
08028 Barcelona, Spain
934021535

Principal investigator: Dr. Ofelia M. Martínez Estrada Postdoctoral Researcher: Dr. Ana García Melero PhD students: Marina Ramiro Pareta • Alejo Torres Cano

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Current
Research

Research interest

The Wilms’ tumor-1 (WT1) gene encodes a zinc finger protein that plays key roles during the formation of mesodermal derived tissues during embryonic development. Interestingly, latest findings indicate that WT1 is also involved in the maintenance of several adult tissues involving cells of mesodermal origin. In both scenarios increased expression of WT1 correlates with the highest plasticity state of the mesodermal progenitor cells.

We are interested in identifying the molecular mechanisms by which WT1 regulates cell plasticity, and how these influence organ formation and regeneration with especial interest in heart development and repair. We use the power of several Wt1 reporter mice and Wt1KO mice combined with the latest molecular and cellular biology tools to address the importance of WT1 in cell plasticity.

Although our research is included in basic science, our results will define the relevance of new pathways involved in cell plasticity and may impact directly in the design of new therapies or strategies for the treatment of cardiovascular disease following ischaemic damage.

Current research lines

Metabolic regulation of epicardium cell plasticity during heart development and repair

Control of epicardium cell shape and plasticity during heart formation and repair

Identification of novel pathways involved in epicardium formation

Our current projects are funded by La Caixa foundation, Health Research 2017; Spanish Society of Cardiology 2017;   La Marató TV3 foundation, Heart diseases 2014; Ministry of Science, Innovation and Universities BFU2015-618135-P

Selected
Publications

Velecela V, Lettice LA, Chau YY, Slight J, Berry RL, Thornburn A, Gunst QD, van den Hoff M, Reina M, Martinez FO, Hastie ND, Martinez-Estrada OM. Wt1 regulates the expression of inhibitory chemokines during heart development. Human molecular genetics. 2013;22:5083-5095

Guadix JA, Ruiz-Villalba A, Lettice L, Velecela V, Munoz-Chapuli R, Hastie ND, Perez-Pomares JM, Martinez-Estrada OM. Wt1 controls retinoic acid signalling in embryonic epicardium through transcriptional activation of raldh2. Development. 2011;138:1093-1097

Martinez-Estrada OM, Lettice LA, Essafi A, Guadix JA, Slight J, Velecela V, Hall E, Reichmann J, Devenney PS, Hohenstein P, Hosen N, Hill RE, Munoz-Chapuli R, Hastie ND. Wt1 is required for cardiovascular progenitor cell formation through transcriptional control of snail and e-cadherin. Nature genetics. 2010;42:89-93

Chau YY, Bandiera R, Serrels A, Martinez-Estrada OM, Qing W, Lee M, Slight J, Thornburn A, Berry R, McHaffie S, Stimson RH, Walker BR, Chapuli RM, Schedl A, Hastie N. Visceral and subcutaneous fat have different origins and evidence supports a mesothelial source. Nature cell biology. 2014;16:367-375

Segarra-Mondejar M, Casellas-Diaz S, Ramiro-Pareta M, Muller-Sanchez C, Martorell-Riera A, Hermelo I, Reina M, Aragones J, Martinez-Estrada OM, Soriano FX. Synaptic activity-induced glycolysis facilitates membrane lipid provision and neurite outgrowth. The EMBO journal. 2018;37

Selected
Publications

Author

Identification

  1. Velecela V, Lettice LA, Chau YY, Slight J, Berry RL, Thornburn A, Gunst QD, van den Hoff M, Reina M, Martinez FO, Hastie ND, Martinez-Estrada OM. Wt1 regulates the expression of inhibitory chemokines during heart development. Human molecular genetics. 2013;22:5083-5095
  2. Guadix JA, Ruiz-Villalba A, Lettice L, Velecela V, Munoz-Chapuli R, Hastie ND, Perez-Pomares JM, Martinez-Estrada OM. Wt1 controls retinoic acid signalling in embryonic epicardium through transcriptional activation of raldh2. Development. 2011;138:1093-1097
  3. Martinez-Estrada OM, Lettice LA, Essafi A, Guadix JA, Slight J, Velecela V, Hall E, Reichmann J, Devenney PS, Hohenstein P, Hosen N, Hill RE, Munoz-Chapuli R, Hastie ND. Wt1 is required for cardiovascular progenitor cell formation through transcriptional control of snail and e-cadherin. Nature genetics. 2010;42:89-93
  4. Chau YY, Bandiera R, Serrels A, Martinez-Estrada OM, Qing W, Lee M, Slight J, Thornburn A, Berry R, McHaffie S, Stimson RH, Walker BR, Chapuli RM, Schedl A, Hastie N. Visceral and subcutaneous fat have different origins and evidence supports a mesothelial source. Nature cell biology. 2014;16:367-375
  5. Segarra-Mondejar M, Casellas-Diaz S, Ramiro-Pareta M, Muller-Sanchez C, Martorell-Riera A, Hermelo I, Reina M, Aragones J, Martinez-Estrada OM, Soriano FX. Synaptic activity-induced glycolysis facilitates membrane lipid provision and neurite outgrowth. The EMBO journal. 2018;37